Exploring the Effects of Optically Active Solvents in Chiral Chromatography on Polysaccharide-Based Columns

• Published in: Analytical chemistry (Washington) 2024-09, Vol.96 (37), p.14720-14726
• Main Authors: Rahmani, Turaj, Lynen, Frédéric
• Format: Article
• Language: English
• Subjects: Acetonitrile; Chromatography; Ethyl lactate; Lactic acid; Liquid chromatography; Low temperature; Methanol; Optical activity; Polysaccharides; Solutes; Solvents; Stereoisomerism; Stereoisomers
• ISSN: 0003-2700; 1520-6882; 1520-6882
• DOI: 10.1021/acs.analchem.3c05040

Exploring the effectiveness of optically active solvents as mobile-phase modifiers in chiral liquid chromatography (LC) can offer an additional new tool to tune the chiral selectivity. Hence, the potential of l-ethyl lactate (LEL), a biobased solvent of this nature, was explored for its distinctive interactions with both the mobile phase and analytes, as anticipated from its chiral nature. The findings reveal that LEL provides distinct selectivity compared to commonly used modifiers in chiral LC. Reversed-phase LC (RPLC)-type chiral separations were therefore compared under various conditions whereby LEL was partially or completely replacing common achiral solvents such as acetonitrile (ACN) and methanol (MeOH). An increase in chiral resolution was obtained in 8 of 16 test compounds. For 5 of them a decrease was obtained, and 3 test solutes did not offer satisfactory results under any of the tested conditions on the polysaccharide columns. When LEL was combined with methanol instead of ACN, worse results were obtained, presumably due to its protic nature. Moreover, LEL demonstrates excellent compatibility with salt additives and is fully miscible with aqueous phases. Interestingly, a steeper increase in chiral resolution is observed for LEL, as compared to ACN at lower temperatures. While LEL is somewhat hindered by its higher UV absorbance, it paves the way toward more simplified chiral screening platforms, whereby chiral solutions can be found for fewer columns and greener solvents such as LEL are incorporated. Finally, to elucidate the impact of chiral interactions between the solvent and analytes, the influence of d-ethyl lactate (DEL) was compared with that of LEL. The results revealed different interactions between the stereoisomers of ethyl lactate (EL) and chiral analytes, demonstrating an influence of optically active solvents on enantioseparations.