Effects of alfa lipoic acid and coenzyme Q10 treatment on AFB1-induced oxidative, inflammatory, and DNA damages in rats

• Published in: Toxicon (Oxford) 2024-10, Vol.249, p.108083, Article 108083
• Main Authors: Albadrani, Ghadeer M., Altyar, Ahmed E., Kensara, Osama A., Haridy, Mohie A.M., Sayed Zaazouee, Mohamed, Ahmed Elshanbary, Alaa, Sayed, Amany A., Abdel-Daim, Mohamed M.
• Format: Article
• Language: English
• Subjects: 8-OHdG; AFB1; ALA; Anti-inflammatory; CQ10; DNA-Damage; Oxidative
• ISSN: 0041-0101; 1879-3150; 1879-3150
• DOI: 10.1016/j.toxicon.2024.108083

Food contamination with Aflatoxin B1 (AFB1) is a worldwide concern that adversely affects animal and human health. The study aimed to evaluate the protective effect of alpha lipoic acid (ALA) and/or co-enzyme Q10 (CQ10) against the harmful effects of AFB1 on the liver and kidneys. Fifty-six mature male Wistar Albino rats (180–200 g) were divided into seven groups, each with eight rats: (1) saline was given as a control, (2) ALA (100 mg/kg bw/day) was given by stomach gavage for fifteen days, and (3) CQ10 (10 mg/kg bw/day) was given by stomach gavage for fifteen days. Group (4) orally given AFB1 (2.5 mg/kg bw) on days 12th and 14th, (5) received AFB1 and ALA, (6) received AFB1 and CQ10, and (7) received AFB1, ALA, and CQ10, as previously described in the ALA, CQ10, and AFB1 groups. After the exposure to AFB1, a significant increase in liver markers (AST, ALT, ALP, and LDH) and renal function tests (BUN and creatinine) was observed compared with the control. ALA and/or CQ10 significantly reduced enzymes of liver and renal functions, as compared with AFB1. AFB1 exposure threw off the balance between oxidants and antioxidants. Still, ALA and/or CQ10 made oxidative stress (MDA, NO, and 8-OHdG) much lower and antioxidant activities (GSH, GSH-Px, SOD, and CAT) much higher. When we used the two together, the activities matched the control levels. Interestingly, this study shows that ALA and CQ10 significantly lowered IL-1β, IL-6, and TNF-α levels compared to the control values when used together after AFB1 exposure caused robust inflammation. Some CQ10 treatment parameters significantly outperformed those of ALA. ALA and CQ10 together worked better than either one alone to protect against AFB1-induced toxicity in the hepatic and renal parenchyma in terms of reducing inflammation, preventing DNA damage, and fighting free radicals. [Display omitted] •Aflatoxin B1 induced hepatorenal oxidative injury and DNA damage.•Alfa lipoic acid ameliorates Aflatoxin B-induced hepatorenal injury and enhances DNA repair.•Co-enzyme Q10 ameliorates Aflatoxin B-induced hepatorenal injury and enhances DNA repair.•Alfa lipoic acid and Co-enzyme Q10 induced potentiating protective effects against aflatoxin B- intoxication.