Transcriptomic analysis of meiotic genes during the mitosis-to-meiosis transition in Drosophila females

• Published in: Genetics (Austin) 2024-10, Vol.228 (2)
• Main Authors: Vallés, Ana Maria, Rubin, Thomas, Macaisne, Nicolas, Dal Toe, Laurine, Molla-Herman, Anahi, Antoniewski, Christophe, Huynh, Jean-René
• Format: Article
• Language: English
• Subjects: Animals; Chromosome Pairing - genetics; Drosophila melanogaster - genetics; Drosophila Proteins - genetics; Drosophila Proteins - metabolism; Endodeoxyribonucleases - genetics; Endodeoxyribonucleases - metabolism; Female; Gene Expression Profiling - methods; Meiosis - genetics; Mitosis - genetics; Transcriptome
• ISSN: 1943-2631; 1943-2631
• DOI: 10.1093/genetics/iyae130

Germline cells produce gametes, which are specialized cells essential for sexual reproduction. Germline cells first amplify through several rounds of mitosis before switching to the meiotic program, which requires specific sets of proteins for DNA recombination, chromosome pairing, and segregation. Surprisingly, we previously found that some proteins of the synaptonemal complex, a prophase I meiotic structure, are already expressed and required in the mitotic region of Drosophila females. Here, to assess if additional meiotic genes were expressed earlier than expected, we isolated mitotic and meiotic cell populations to compare their RNA content. Our transcriptomic analysis reveals that all known meiosis I genes are already expressed in the mitotic region; however, only some of them are translated. As a case study, we focused on mei-W68, the Drosophila homolog of Spo11, to assess its expression at both the mRNA and protein levels and used different mutant alleles to assay for a premeiotic function. We could not detect any functional role for Mei-W68 during homologous chromosome pairing in dividing germ cells. Our study paves the way for further functional analysis of meiotic genes expressed in the mitotic region.