The Fat Body-Specific GST Gene SlGSTe11 Enhances the Tolerance of Spodoptera litura to Cyantraniliprole and Nicotine

• Published in: Journal of agricultural and food chemistry 2024-09, Vol.72 (36), p.19680-19688
• Main Authors: Jin, Long, Yan, Kunpeng, Kong, Haoran, Li, Jianyi, Fan, Chengcheng, Pan, Yiou, Shang, Qingli
• Format: Article
• Language: English
• Subjects: Agricultural and Environmental Chemistry; Animals; Fat Body - drug effects; Fat Body - enzymology; Fat Body - metabolism; Glutathione Transferase - chemistry; Glutathione Transferase - genetics; Glutathione Transferase - metabolism; Insect Proteins - chemistry; Insect Proteins - genetics; Insect Proteins - metabolism; Insecticide Resistance - genetics; Insecticides - chemistry; Insecticides - metabolism; Insecticides - pharmacology; Molecular Docking Simulation; Nicotine - metabolism; ortho-Aminobenzoates - metabolism; ortho-Aminobenzoates - pharmacology; Pyrazoles - pharmacology; Spodoptera - drug effects; Spodoptera - enzymology; Spodoptera - genetics; Spodoptera - growth & development; Spodoptera - metabolism
• ISSN: 0021-8561; 1520-5118; 1520-5118
• DOI: 10.1021/acs.jafc.4c05747

Spodoptera litura is a significant agricultural pest, and its glutathione S-transferase (GST) plays a crucial role in insecticide resistance. This study aimed to investigate the relationship between the SlGSTe11 gene of S. litura and resistance to cyantraniliprole and nicotine. Transcriptome analysis revealed that SlGSTe11 is highly expressed mainly in fat bodies, with a significant increase in SlGSTe11 gene expression under induction by cyantraniliprole and nicotine. The ectopic expression of the SlGSTe11 gene in transgenic fruit flies resulted in a 5.22-fold increase in the tolerance to cyantraniliprole. Moreover, compared to the UAS-SlGSTe11 line, the Act5C-UAS>SlGSTe11 line laid more eggs and had a lower mortality after nicotine exposure. RNAi-mediated inhibition of SlGSTe11 gene expression led to a significant increase in the mortality of S. litura under cyantraniliprole exposure. In vitro metabolism experiments demonstrated that the recombinant SlGSTe11 protein efficiently metabolizes cyantraniliprole. Molecular docking results indicated that SlGSTe11 has a strong affinity for both cyantraniliprole and nicotine. These findings suggest that SlGSTe11 is involved in the development of resistance to cyantraniliprole and nicotine in S. litura.