Vangl2 regulates intercellular junctions by remodeling actin-based cytoskeleton through the Rock signaling pathway during spermatogenesis in Eriocheir sinensis

• Published in: International journal of biological macromolecules 2024-11, Vol.279 (Pt 2), p.135264, Article 135264
• Main Authors: Zhao, Zhan, Qi, Hong-Yu, Li, Zhen-Fang, Wang, Lan-Min, Wang, Jia-Ming, Tan, Fu-Qing, Yang, Wan-Xi
• Format: Article
• Language: English
• Subjects: Eriocheir sinensis; Intercellular junction; Rock signaling; Spermatogenesis; Vangl2
• ISSN: 0141-8130; 1879-0003; 1879-0003
• DOI: 10.1016/j.ijbiomac.2024.135264

As a key planar cell polarity protein, Van Gogh-like 2 (Vangl2) is essential for mammalian spermatogenesis. As a decapod crustacean, Eriocheir sinensis exhibits distinct spermatogenic processes due to its unique seminiferous tubule morphology and hemolymph-testis barrier (HTB). To determine whether Vangl2 performs analogous functions in E. sinensis, we identified the Es-Vangl2. Es-Vangl2 exhibited high expression and wide distribution in the testes, indicating its crucial involvement in spermatogenesis. Following targeted knockdown of Es-Vangl2in vivo, the structure of seminiferous tubules was disrupted, characterized by vacuolization of the germinal zone and obstruction of spermatozoon release. Concurrently, the integrity of the HTB was compromised, accompanied by reduced expression and aberrant localization of junction proteins. More importantly, the regulatory influence of Es-Vangl2 was manifested through modulating the organization of microfilaments, a process mediated by epidermal growth factor receptor pathway substrate 8 (Eps8). Further studies demonstrated that these phenotypes resulting from Es-Vangl2 knockdown were attributed to the inhibition of Rock signaling pathway activity, which was verified by the Es-Rock interference and Y27632 inhibition assays. In summary, the findings highlight the pivotal role of Es-Vangl2 in stabilizing HTB integrity by regulating Eps8-mediated actin remodeling through the Rock signaling pathway in the spermatogenesis of E. sinensis. •Es-Vangl2 is characterized for the first time.•Es-Vangl2 participates in the maintenance of testis morphology and function.•Knockdown of Es-Vangl2 destroys the integrity of HTB.•Deficient Es-Vangl2 inhibits the Rock signaling pathway.•Decreased Rock activity downstream of Es-Vangl2 leads to F-actin disorganization.