Molecular epidemiology of brucellosis in Asia: insights from genotyping analyses

• Published in: Veterinary research communications 2024-12, Vol.48 (6), p.3533-3550
• Main Authors: Ali, Shahzad, Mushtaq, Areeba, Hassan, Laiba, Syed, Muhammad Ali, Foster, Jeffrey T., Dadar, Maryam
• Format: Article
• Language: English
• Subjects: Animals; Asia - epidemiology; Biomedical and Life Sciences; Brucella; Brucella abortus - classification; Brucella abortus - genetics; Brucella abortus - isolation & purification; Brucella melitensis - classification; Brucella melitensis - genetics; Brucellosis; Brucellosis - epidemiology; Brucellosis - microbiology; Brucellosis - veterinary; Epidemiology; Genetic diversity; Genetic relationship; Genomic analysis; Genotype; Genotypes; Genotyping; Humans; Life Sciences; Livestock; Minisatellite Repeats - genetics; Molecular Epidemiology; Multilocus sequence typing; Multilocus Sequence Typing - veterinary; Nomenclature; Nucleotide sequence; Phylogeny; Review; Strains (organisms); Terminology; Veterinary Medicine/Veterinary Science; Whole genome sequencing; Zoology
• ISSN: 0165-7380; 1573-7446; 1573-7446
• DOI: 10.1007/s11259-024-10519-5

Brucellosis infects humans and animals worldwide but is particularly prevalent in Asia. In many Asian countries, molecular diagnostic tools for accurate molecular diagnostics and molecular epidemiology are lacking. Nonetheless, some countries have conducted in-depth molecular epidemiological studies. The objective of this study was to reveal the genetic relationships, geographic origins, and distributions of Brucella strains across Asia for two primary species, B. abortus and B. melitensis . For this, we systematically searched genotyping data from published studies on the molecular epidemiology of Brucella species for both humans and livestock in Asia. We used data from multilocus sequence typing (MLST), multiple-locus variable-number tandem repeat analysis (MLVA), and whole genome sequencing analysis of Brucella strains. We also analyzed the MLVA genotypes of 129 B. abortus isolates and 242 B. melitensis isolates with known origins in Asia from an online MLVA database using MLVA-11 data in minimum spanning trees and MLVA-16 data in neighbor-joining trees. We found that the B. melitensis East Mediterranean lineage is predominant across the continent, with only a small number of samples from the Africa and Americas lineages, and none from the West Mediterranean lineage. The “abortus C” genotype was the most common group of B. abortus in Asia, with limited genetic variation for this species. Several studies also reported that Near Eastern countries frequently encounter human brucellosis cases of B. abortus from genotypes 42 and 43. Our study highlights the inconsistent collection of genetic data for Brucella species across Asia and a need for more extensive sampling in most countries. Finally, a consistent nomenclature is necessary to define various groupings of strains within a lineage (i.e., clade) so uniform terminology should denote particular genetic groups that are understood by all researchers.