TRAF3IP3 Blocks Mitophagy to Exacerbate Myocardial Injury Induced by Ischemia–Reperfusion

• Published in: Cardiovascular toxicology 2024-11, Vol.24 (11), p.1204-1214
• Main Authors: Wei, Zhongcheng, Liu, Juan, Liu, Hailang, Jiang, Aixia
• Format: Article
• Language: English
• Subjects: Animals; Antibodies; Biomedical and Life Sciences; Biomedicine; Cardiology; Cardiomyocytes; Cardiovascular disease; Cell culture; Cell death; Cell Line; Disease Models, Animal; Flow cytometry; Glioma; Heart attacks; Ischemia; Kinases; Male; Membrane Proteins - genetics; Membrane Proteins - metabolism; Membranes; Mitochondria; Mitochondria, Heart - metabolism; Mitochondria, Heart - pathology; Mitophagy; Myocardial infarction; Myocardial Infarction - genetics; Myocardial Infarction - metabolism; Myocardial Infarction - pathology; Myocardial Reperfusion Injury - genetics; Myocardial Reperfusion Injury - metabolism; Myocardial Reperfusion Injury - pathology; Myocardial Reperfusion Injury - prevention & control; Myocytes, Cardiac - metabolism; Myocytes, Cardiac - pathology; Nedd4 protein; Pharmacology/Toxicology; Proteins; Proteolysis; Rats; Rats, Sprague-Dawley; Reperfusion; Signal Transduction; Toxicology; Ubiquitin-Protein Ligases - genetics; Ubiquitin-Protein Ligases - metabolism
• ISSN: 1530-7905; 1559-0259; 1559-0259
• DOI: 10.1007/s12012-024-09916-8

To uncover the possible role of TRAF3IP3 in the progression of myocardial infarction (MI), clarify its role in mitophagy and mitochondrial function, and explore the underlying mechanism. GEO chip analysis, RT-qPCR, and LDH release assay were used to detect the expression of TRAF3IP3 in tissues and cells and its effects on cell damage. Immunostaining and ATP product assays were performed to examine the effects of TRAF3IP3 on mitochondrial function. Co-IP, CHX assays, Immunoblot and Immunostaining assays were conducted to determine the effects of TRAF3IP3 on mitophagy. TRAF3IP3 was highly expressed in IR rats and HR-induced H9C2 cells. TRAF3IP3 knockdown can alleviate H/R-induced H9C2 cell damage. In addition, TRAF3IP3 knockdown can induce mitophagy, thus enhancing mitochondrial function. We further revealed that TRAF3IP3 can promote the degradation of NEDD4 protein. Moreover, TRAF3IP3 knockdown suppressed myocardial injury in I/R rats. TRAF3IP3 blocks mitophagy to exacerbate myocardial injury induced by I/R via mediating NEDD4 expression.