Isolation of lipase producing Bacillus sp. from olive mill wastewater and improving its enzyme activity

• Published in: Journal of hazardous materials 2007-11, Vol.149 (3), p.720-724
• Main Authors: Ertugrul, Sevgi, Donmez, Gonul, Takac, Serpil
• Format: Article
• Language: English
• Subjects: Agar - chemistry; Applied sciences; Bacillus - enzymology; Bacillus - metabolism; Bacillus sp; Biological and medical sciences; Culture Media - chemistry; Enzymes - chemistry; Exact sciences and technology; Fatty Acids - chemistry; Food engineering; Food industries; Fundamental and applied biological sciences. Psychology; General aspects; General purification processes; Hydrogen-Ion Concentration; Industrial Microbiology - methods; Industrial Waste; Isolation; Lipase; Lipase - chemistry; Lipolysis; Olea; Olea - chemistry; Olive mill wastewater; Pollution; Temperature; Time Factors; Triglycerides - chemistry; Waste Disposal, Fluid - methods; Wastewaters; Water treatment and pollution
• ISSN: 0304-3894; 1873-3336
• DOI: 10.1016/j.jhazmat.2007.04.034

The bacteria that could grow on media containing olive mill wastewater (OMW) were isolated and their lipase production capacities were investigated. The strain possessing the highest lipase activity among 17 strains grown on tributyrin agar medium was identified as Bacillus sp. The effect of initial pH on the lipase activity was investigated in tributyrin medium and pH 6 was found to be the optimal. The liquid medium composition was improved by replacing tributyrin with various carbon sources. Among the media containing different compositions of triolein, trimyristin, trilaurin, tricaprin, tricaprylin, tributyrin, triacetin, Tween 80, OMW, glucose, and whey; the medium contained 20% whey +1% triolein was found to give the highest lipase activity. Cultivation of Bacillus sp. in the optimal medium at pH 6 and 30 °C for 64 h resulted in the extracellular and intracellular lipase activities of 15 and 168 U/ml, respectively.