Impacts of phosphoenolpyruvate carboxylase gene silencing on photosynthetic efficiency and carbon fixation in Skeletonema costatum

• Published in: Gene 2025-01, Vol.933, p.148915, Article 148915
• Main Authors: Zhen, Yu, Zhu, Jiwei, Yue, Ming, Mi, Tiezhu
• Format: Article
• Language: English
• Subjects: Carbon - metabolism; Carbon Cycle - genetics; Carbon fixation; Diatoms - genetics; Diatoms - metabolism; Gene Silencing; Phosphoenolpyruvate carboxylase; Phosphoenolpyruvate Carboxylase - genetics; Phosphoenolpyruvate Carboxylase - metabolism; Photosynthesis - genetics; Skeletonema costatum
• ISSN: 0378-1119; 1879-0038; 1879-0038
• DOI: 10.1016/j.gene.2024.148915

•PEPC gene-silenced strains of Skeletonema costatum were constructed.•Silencing the PEPC gene did not significantly impact cell growth, but it did inhibit photosynthesis.•Silencing the PEPC gene downregulates gene expression and reduces enzyme activities related to the C4 pathway.•Silencing the PEPC gene has little effect on the gene expression and activity of Rubisco but upregulates those of carbonic anhydrase. Diatoms play a crucial role in marine primary productivity through carbon fixation, which is essential for understanding the operation of marine biological pumps and carbon sinks. This study focuses on the phosphoenolpyruvate carboxylase (PEPC) gene, a key enzyme in the carbon assimilation pathway of diatoms, by investigating the consequences of its silencing in Skeletonema costatum. Through this approach, we aimed to clarify the distinct contributions of PEPC to the overall carbon fixation process. The mutant strains of S. costatum were subjected to thorough analysis to identify any shifts in physiological behavior, alterations in the gene expression of key carbon-fixing enzymes, and changes in the associated enzyme activities. Notably, the inhibition of the PEPC gene did not significantly affect the growth rate of S. costatum; however, it did have a notable impact on the photosynthetic apparatus, as evidenced by a reduction in the maximal electron transport rate and a decline in light utilization efficiency. A significant decrease was observed in both the enzymatic activity and gene expression of PEPCase. This down-regulation also affected other enzymes integral to the carbon fixation pathway, such as phosphoenolpyruvate carboxykinase and pyruvate-phosphate dikinase, indicating a wider metabolic perturbation. In contrast, the expression and activity of the Rubisco enzyme suggested that some facets of carbon fixation remained resilient. Furthermore, the substantial upregulation of carbonic anhydrase expression and activity probably represented an adaptive mechanism to sustain the inorganic carbon supply necessary for the carboxylation process of Rubisco. This research not only underscores the pivotal role of the PEPC gene in the carbon fixation of S. costatum but also expands our comprehension of carbon fixation mechanisms in diatoms.