Mapping high-risk areas for Mycobacterium tuberculosis complex bacteria transmission: Linking host space use and environmental contamination

• Published in: The Science of the total environment 2024-11, Vol.953, p.176053, Article 176053
• Main Authors: Ferreira, Eduardo M., Cunha, Mónica V., Duarte, Elsa L., Mira, António, Pinto, Daniela, Mendes, Inês, Pereira, André C., Pinto, Tiago, Acevedo, Pelayo, Santos, Sara M.
• Format: Article
• Language: English
• Subjects: Camera-trapping; Environmental contamination; Mycobacterium bovis; Mycobacterium tuberculosis complex (MTBC); Space use; Transmission risk; Wildlife-cattle interface
• ISSN: 0048-9697; 1879-1026; 1879-1026
• DOI: 10.1016/j.scitotenv.2024.176053

In many Mediterranean ecosystems, animal tuberculosis (TB), caused by Mycobacterium bovis, an ecovar of Mycobacterium tuberculosis complex (MTBC), is maintained by multi-host communities. It is hypothesised that interspecies transmission is mainly indirect via shared contaminated environments. Therefore, identifying spatial areas where MTBC bacteria occur and quantifying space use by susceptible hosts might help predict the spatial likelihood of transmission across the landscape. Here, we aimed to evaluate the transmission risk of MTBC in a multi-host system involving wildlife (ungulates and carnivores) and cattle (Bos taurus). We collected eighty-nine samples from natural substrates (water, soil, and mud) at 38 sampling sites in a TB endemic area within a Mediterranean agroforestry system in Portugal. These samples were analysed by real-time PCR to detect MTBC DNA. Additionally, host-specific space use intensity maps were obtained through camera-trapping covering the same sampling sites. Results evidenced that a significant proportion of samples were positive for MTBC DNA (49 %), suggesting that the contamination is widespread in the area. Moreover, they showed that the probability of MTBC occurrence in the environment was significantly influenced by topographic features (i.e., slope), although other non-significant predictor related with soil conditions (SMI: soil moisture index) incorporated the MTBC contamination model. The integration of host space use intensity maps with the spatial detection of MTBC showed that the red deer (Cervus elaphus) and wild boar (Sus scrofa) exhibited the highest percentages of high-risk areas for MTBC transmission. Furthermore, when considering the co-occurrence of multiple hosts, transmission risk analyses revealed that 26.5 % of the study area represented high-risk conditions for MTBC transmission, mainly in forest areas. [Display omitted] •The ecological background is seldomly incorporated in the investigation of non-vector-based disease systems.•Host ecology and environmental contamination data were linked to quantify spatial transmission risk of MTBC in a multi-host system.•Red deer and wild boar are associated to the highest-risk areas for MTBC transmission.•One-quarter of the study area represents high transmission risk when considering a multi-host system.