Selenium Treatment Ameliorates the Adverse Effects Caused by Dynamin Gene Knockdown in Bombyx mori

• Published in: Archives of insect biochemistry and physiology 2024-09, Vol.117 (1), p.e22148-n/a
• Main Authors: Hong, Ting‐Ting, Hu, Shuo, Hu, Fei, Ge, Wen‐Jie, Thakur, Kiran, Tang, Shun‐Ming, Wei, Zhao‐Jun
• Format: Article
• Language: English
• Subjects: Animals; Body weight; Bombyx - drug effects; Bombyx - genetics; Bombyx - growth & development; Bombyx - metabolism; Bombyx mori; Catalase; Cocoons; Dynamin; Dynamins - genetics; Dynamins - metabolism; Eggs; Endocytosis; Female; Fertility; Gene expression; Gene Knockdown Techniques; Glutathione; Insect Proteins - genetics; Insect Proteins - metabolism; Larva - drug effects; Larva - genetics; Larva - growth & development; Larva - metabolism; Physiological effects; Proteins; RNA Interference; RNA, Small Interfering - genetics; RNA, Small Interfering - metabolism; RNA-mediated interference; Selenium; Selenium - pharmacology; Side effects; Silk; Silk gland; Silkworms; siRNA; siRNAs; Superoxide dismutase
• ISSN: 0739-4462; 1520-6327; 1520-6327
• DOI: 10.1002/arch.22148

ABSTRACT Our previous research reported the influence of 50 μM selenium (Se) on the cytosolization (endocytosis) pathway, which in turn stimulates the growth and development of Bombyx mori. Lately, dynamin is recognized as one of the key proteins in endocytosis. To explore the underlying mechanisms of Se impact, the dynamin gene was knocked down by injecting siRNAs (Dynamin‐1, Dynamin‐2, and Dynamin‐3). This was followed by an analysis of the target gene and levels of silk protein genes, as well as growth and developmental indices, Se‐enrichment capacity, degree of oxidative damage, and antioxidant capacity of B. mori. Our findings showed a considerable decrease in the relative expression of the dynamin gene in all tissues 24 h after the interference and a dramatic decrease in the silkworm body after 48 h. RNAi dynamin gene decreased the silkworm body weight, cocoon shell weight, and the ratio of cocoon. In the meantime, malondialdehyde level increased and glutathione level and superoxide dismutase/catalase activities decreased. 50 μM Se markedly ameliorated these growth and physiological deficits as well as decreases in dynamin gene expression. On the other hand, there were no significant effects on fertility (including produced eggs and laid eggs) between the interference and Se treatments. Additionally, the Se content in the B. mori increased after the dynamin gene interference. The dynamin gene was highly expressed in the silk gland and declined significantly after interference. Among the three siRNAs (Dynamin‐1, Dynamin‐2, and Dynamin‐3), the dynamin‐2 displayed the highest interference effects to target gene expression. Our results demonstrated that 50 μM Se was effective to prevent any adverse effects caused by dynamin knockdown in silkworms. This provides practical implications for B. mori breeding industry. Present research demonstrated that 50 μM Selenium (Se) treatment alleviated dynamin gene knockdown. Dynamin gene knockdown enriched Se content in the Bombyx mori. Dynamin mRNA expression was highest in the silk gland, suggesting its role in silk production. Summary 50 μM Selenium (Se) treatment alleviated any adverse effects on Bombyx mori development caused by dynamin gene knockdown. Dynamin gene knockdown enriched Se content in B. mori. Dynamin was highly expressed in the silk gland, suggesting its role in silk production.