Segmental patterning of microbiota and immune cells in the murine intestinal tract

The intestine exhibits distinct characteristics along its length, with a substantial immune cell reservoir and diverse microbiota crucial for maintaining health. This study investigates how anatomical location and regional microbiota influence intestinal immune cell abundance. Using conventionally c...

Full description

Saved in:
Bibliographic Details
Published in:Gut microbes 2024-12, Vol.16 (1), p.2398126
Main Authors: Anandakumar, Harithaa, Rauch, Ariana, Wimmer, Moritz I., Yarritu, Alex, Koch, Gudrun, McParland, Victoria, Bartolomaeus, Hendrik, Wilck, Nicola
Format: Article
Language:English
Subjects:
Animals
Bacteria - classification
Bacteria - genetics
Bacteria - immunology
Bacteria - isolation & purification
Female
Gastrointestinal Microbiome
Germ-Free Life
germ-free vs colonized
immune cells
Intestinal Mucosa - cytology
Intestinal Mucosa - immunology
Intestinal Mucosa - microbiology
Intestines - cytology
Intestines - immunology
Intestines - microbiology
Male
Metagenomics
Mice
Mice, Inbred C57BL
Microbiome
Research Paper
segmental patterning
T-Lymphocyte Subsets - immunology
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The intestine exhibits distinct characteristics along its length, with a substantial immune cell reservoir and diverse microbiota crucial for maintaining health. This study investigates how anatomical location and regional microbiota influence intestinal immune cell abundance. Using conventionally colonized and germ-free mice, segment-specific immune cell composition and microbial communities were assessed. Metagenomic sequencing analyzed microbiome variations, while flow cytometry and immunofluorescence examined immune cell composition. Microbiome composition varied significantly along the intestine, with diversity and abundance increasing from upper to lower segments. Immune cells showed distinct segment-specific patterning influenced by microbial colonization and localization. T cell subsets displayed varied dependence on microbiome presence and anatomical location. This study highlights locoregional differences in intestinal immune cell and microbiome composition, identifying immune subsets susceptible to microbiota presence. The findings provide context for understanding immune cell alterations in disease models.
ISSN:1949-0976
1949-0984
1949-0984
DOI:10.1080/19490976.2024.2398126