Poly-tannic acid coated PLGA nanoparticle decorated with antimicrobial peptide for synergistic bacteria treatment and infectious wound healing promotion

• Published in: Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2025-01, Vol.245, p.114217, Article 114217
• Main Authors: Guo, Mingxue, Ruan, Mingyue, Wu, Jiamin, Ye, Jinhong, Wang, Changhai, Guo, Zishuo, Chen, Wanling, Wang, Liu, Wu, Kai, Du, Shouying, Han, Ning, Lu, Yang
• Format: Article
• Language: English
• Subjects: Animals; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Antibacterial; antimicrobial peptides; Antimicrobial Peptides - chemistry; Antimicrobial Peptides - pharmacology; Escherichia coli - drug effects; Mice; Microbial Sensitivity Tests; Nanoparticles - chemistry; Particle Size; Photothermal; Polylactic Acid-Polyglycolic Acid Copolymer - chemistry; Polylactic Acid-Polyglycolic Acid Copolymer - pharmacology; Polyphenols; Polytannic acid; Staphylococcus aureus - drug effects; Surface Properties; Wound healing; Wound Healing - drug effects
• ISSN: 0927-7765; 1873-4367; 1873-4367
• DOI: 10.1016/j.colsurfb.2024.114217

Bacterial infections pose a great threat to human health. Therefore, the development of new antibacterial agents or methods is in urgent need. In this study, we prepared polytannic acid (pTA)-coated PLGA nanoparticles decorated with Dermaseptin-PP (Der), an antimicrobial peptide (AMP), on the surface to obtain PLGA-pTA-Der. This nanoplatform could combine AMPs with photothermal treatment (PTT) mediated by pTA to achieve synergistic bacterial killing. The results of in vitro experiments showed that the PLGA-pTA-Der nanoparticles could eliminate nearly 99 % of Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) upon near-infrared (NIR) laser irradiation (2.0 W·cm−2, 5 min), demonstrating excellent antibacterial properties. In addition, the results of atomic force microscopy (AFM) revealed that PLGA-pTA-Der with laser irradiation can greatly destroy the mechanical integrity of the bacterial outer membrane. And the presence of Der could exacerbate the heat damage caused by the PLGA-pTA NPs to the bacteria, which is helpful to reduce the critical temperature required for bacteria killing by PTT. In vivo experiments showed that PLGA-pTA-Der nanoparticles with laser irradiation significantly accelerated the wound healing process and inhibited the growth of bacterial. Moreover, it can achieve a strong photothermal antibacterial effect at a mild temperature (