Endocytosis of Synaptic Vesicle in Motor Nerve Endings of FUS Transgenic Mice with a Model of Amyotrophic Lateral Sclerosis

• Published in: Bulletin of experimental biology and medicine 2024-08, Vol.177 (4), p.449-453
• Main Authors: Grigoryev, P. N., Gaptrakhmanova, G. A., Plotnikova, A. A., Zefirov, A. L., Mukhamedyarov, M. A.
• Format: Article
• Language: English
• Subjects: Amyotrophic lateral sclerosis; Biomedical and Life Sciences; Biomedicine; Cell Biology; Disease; Dyes; Endocytosis; Experiments; Fluorescent indicators; Internal Medicine; Laboratory animals; Laboratory Medicine; Medical research; Nerve endings; Neurotransmitter release; Pathology; Physiology; Software; Synaptic vesicles; Transgenic animals; Transgenic mice; Transmitters
• ISSN: 0007-4888; 1573-8221; 1573-8221
• DOI: 10.1007/s10517-024-06206-9

In experiments on the motor nerve endings of the diaphragm of transgenic FUS mice with a model of amyotrophic lateral sclerosis at the pre-symptomatic stage of the disease, the processes of transmitter release and endocytosis of synaptic vesicles were studied. In FUS mice, the intensity of transmitter release during high-frequency stimulation of the motor nerve (50 imp/sec) was lowered. At the same duration of stimulation, the loading of fluorescent dye FM1-43 was lower in FUS mice. However, at the time of stimulation, during which an equal number of quanta are released in wild-type and FUS mice, no differences in the intensity of dye loading were found. Thus, endocytosis is not the key factor in the mechanism of synaptic dysfunction in FUS mice at the pre-symptomatic stage.