Aurantiochytrium mutant strains exhibiting different colony colors altered the contents of squalene

Abstract Aurantiochytrium sp. 18W-13a, a marine heterotrophic protist belonging to the genus thraustochytrid, is known to accumulate high levels of squalene and carotenoids. Nowadays, the mutagenesis breeding of microorganisms is still widely practiced because the induced mutations of DNA do not inv...

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Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2024-11, Vol.88 (12), p.1424-1431
Main Authors: Yang, Tianjing, Kubo, Shohei, Juntila, Darryl Joy, Iwata, Yasushi, Tomita, Kanako, Hase, Yoshihiro, Oono, Yutaka, Araie, Hiroya, Yoneda, Kohei, Maeda, Yoshiaki, Suzuki, Iwane
Format: Article
Language:English
Subjects:
Carotenoids - metabolism
Color
Mutagenesis
Mutation
Squalene - metabolism
Stramenopiles - genetics
Stramenopiles - metabolism
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Summary:Abstract Aurantiochytrium sp. 18W-13a, a marine heterotrophic protist belonging to the genus thraustochytrid, is known to accumulate high levels of squalene and carotenoids. Nowadays, the mutagenesis breeding of microorganisms is still widely practiced because the induced mutations of DNA do not involve the permanent integration of heterologous DNA sequences. Therefore, in this study, we focused on the improvement of squalene yield by mutagenesis breeding using Aurantiochytrium sp. 18W-13a. To bypass the massively laborious screening, we propose to use colony colors as the first criterion to screen mutants with high squalene accumulation, since the carotenoid and squalene synthetic pathways share an intermediate. We selected pale (white)-colored mutants after carbon ion irradiation. The white mutants exhibited larger squalene yields than twice as much of the original strain. The results clearly indicate that the present screening method with colony colors promises to obtain productive strains of squalene. Graphical Abstract Graphical Abstract Due to the selection by colony colors, strains of Aurantiochytrium sp. 18W-13a, which contain high squalene were isolated.
ISSN:1347-6947
1347-6947
DOI:10.1093/bbb/zbae128