Smartphone-assisted fluorescent microfluidic-chip for sensitive detection of sweat glucose via dual-sensing of O2/H2O2

• Published in: Talanta (Oxford) 2025-01, Vol.281, p.126883, Article 126883
• Main Authors: Li, Zhen, Li, Tianyi, Wang, Xiaohui, Ping, Jiantao, Peng, Hongshang
• Format: Article
• Language: English
• Subjects: Dual-sensing; Fluorescent probe; Glucose detection; Microfluidic chip; Smartphone
• ISSN: 0039-9140; 1873-3573; 1873-3573
• DOI: 10.1016/j.talanta.2024.126883

A novel smartphone-assisted fluorescent microfluidic-chip was designed for detecting sweat glucose. The microfluidic chip contained six microchambers, each of which was equipped with a glucose sensing membrane incorporating glucose oxidase (GOD), fluorescent O2 probe PtTFPP and H2O2 probe G1. Based upon O2 consumption and H2O2 generation during glucose catalysis by GOD, the chip produced two fluorescence signals towards glucose under single-wavelength excitation, i.e. green fluorescence in response to H2O2 and red fluorescence to O2. The limit of detection (LOD) based on H2O2 monitoring was 0.005 mM, while the LOD based on O2 monitoring was 0.04 mM. Furthermore, the obtained chip was integrated with a smartphone-based portable platform to record RGB values for point-of-care testing of sweat glucose. Glucose calibration (Y = −3.45 + 1.81∗R + 0.68∗G) at 6-min time point was performed by combining R and G channels signals. The dual-monitoring analysis provided a more accurate and reliable verification of glucose detection. This smartphone-assistant optical microfluidic-chip device holds significant potential for portable self-management of glucose in personalized healthcare and clinical diagnosis. [Display omitted] •A smartphone-assisted fluorescent microfluidic-chip is designed for sweat glucose.•The glucose levels are detected sensitively by dual-monitoring the O2 and H2O2 changes.•This dual-monitoring assay improves the overall detection limit by utilizing the H2O2 responses (0.005 mM).•Glucose calibration combining R and G channels signal, provides more accurate and reliable results.