Assessment of cell death in the liver of Labeo rohita on exposure to an emerging contaminant aspirin: an immunofluorescent, flow cytometric and biochemical investigation

• Published in: Fish physiology and biochemistry 2024-12, Vol.50 (6), p.2551-2562
• Main Authors: Gayen, Tuhina, Tripathi, Anchal, Mittal, Swati, Kumari, Usha
• Format: Article
• Language: English
• Subjects: Animal Anatomy; Animal Biochemistry; Animal Physiology; Animals; Anti-inflammatory agents; Anti-Inflammatory Agents, Non-Steroidal - toxicity; Apoptosis; Apoptosis - drug effects; Aquatic environment; Aspirin; Aspirin - pharmacology; Aspirin - toxicity; Biomedical and Life Sciences; Caspase 3 - metabolism; Caspase-3; Cell death; Cell Death - drug effects; Contaminants; Cyprinidae; Exposure; Fish; Flow Cytometry; Freshwater & Marine Ecology; Hepatocytes; Histology; Kupffer cells; Labeo rohita; Life Sciences; Liver; Liver - drug effects; Morphology; Mortality; Necrosis; Nonsteroidal anti-inflammatory drugs; Nontarget organisms; Prostaglandin endoperoxide synthase; Prostaglandin-Endoperoxide Synthases - metabolism; Toxicity; Tumor Necrosis Factor-alpha - metabolism; Water Pollutants, Chemical - toxicity; Zoology
• ISSN: 0920-1742; 1573-5168; 1573-5168
• DOI: 10.1007/s10695-024-01405-7

Aspirin is one of the most frequently detected non-steroidal anti-inflammatory drugs in aquatic environments. Despite its prevalence, toxicity possessed by aspirin to non-target organisms like fish is poorly explored. In the present study, cell death induced by different concentrations of aspirin (1, 10, and 100 µg/L) has been investigated in the liver of fish, Labeo rohita exposed for 28 days. A significant increase ( p