Maximizing saffron apocarotenoid production in varied tomato fruit carotenoid contexts

• Published in: The Plant journal : for cell and molecular biology 2024-11, Vol.120 (3), p.966-983
• Main Authors: Lobato‐Gómez, Maria, Drapal, Margit, Fernández‐Muñoz, Rafael, Presa, Silvia, Espinosa, Ana, Fraser, Paul D., Gómez‐Gómez, Lourdes, Orzaez, Diego, Granell, Antonio
• Format: Article
• Language: English
• Subjects: Carotene; Carotenoids; Cleavage; Crop yield; Dioxygenase; Fruits; Genotypes; Lycopene; metabolic engineering; Mutation; Optimization; Plant layout; Saffron; saffron apocarotenoids; tomato; Tomatoes; Transgenic plants; Zeaxanthin; β-Carotene
• ISSN: 0960-7412; 1365-313X; 1365-313X
• DOI: 10.1111/tpj.17030

SUMMARY Saffron spice owes its commercial appreciation to its specific apocarotenoids: crocins, picrocrocin, and safranal. In Crocus sativus, these compounds are biosynthesized from zeaxanthin through oxidative cleavage by the carotenoid cleavage dioxygenase 2 (CCD2). Transgenic tomato plants expressing CsCCD2 in the fruit, named Tomaffron, accumulate high levels of saffron apocarotenoids despite the low substrate availability for CsCCD2. In the present study, CsCCD2 has been introduced into Xantomato; this tomato variety accumulates high levels of zeaxanthin and β‐carotene in ripe fruit due to a combination of four mutant alleles. Xantomato and Tomaffron genotypes have been combined to optimize apocarotenoid production. The best transgenic lines accumulated 15 and 14 times more crocins and picrocrocin than Tomaffron, alongside a fourfold increase in β‐carotene compared to Xantomato, albeit at a cost in fruit yield. Segregation of the four mutations has been carried out to find the best combination for obtaining high levels of saffron apocarotenoids without adverse effects on fruit yield. Plants harboring the high‐pigmented 3 (hp3) and BETA (BSh) mutations accumulated 6 and 15 times more crocins and picrocrocin than Tomaffron, without observable pleiotropic effects. Additionally, those high levels of saffron apocarotenoids were obtained in fruit accumulating high levels of both lycopene and β‐carotene independently or in combination, suggesting a regulatory role for the apocarotenoids produced and indicating that it is possible to increase the levels of both types of healthy promoting molecules simultaneously. Significance Statement Our study introduces a method to significantly boost saffron apocarotenoid levels in tomato fruit, while maintaining fruit yield. This innovation not only revolutionizes crocin and picrocrocin production but also highlights the potential for simultaneous accumulation of saffron apocarotenoids and carotenoids, offering promising prospects for agriculture and nutrition.