Phytochemical, spectroscopic analysis and antifungal activity on bell peppers of hydrothermal bioactive metabolites of Humulus lupulus L. extracts

• Published in: Natural product research 2024-09, p.1-12
• Main Authors: Basilio-Cortes, Ulin Antobelli, Ramírez-Rodrigues, Milena M, Ramírez-Rodrigues, Melissa A, González-Mendoza, Daniel, Tzintzun-Camacho, Olivia, Durán-Hernández, Dagoberto, González-Salitre, Lourdes
• Format: Article
• Language: English
• ISSN: 1478-6419; 1478-6427; 1478-6427
• DOI: 10.1080/14786419.2024.2405010

This study aimed to evaluate the impact of temperature on the potential extraction of bioactive compounds from aqueous hop extract samples. The main bioactive components were characterised and analysed by LC-MS/MS, FT-IR, phenolic compounds and total flavonoids. Antifungal activity was evaluated and in bell peppers. LC-MS/MS analysis demonstrated increases and decreases of bioactive compounds in both extracts depending on the extraction temperature of 25 or 65 °C. The bioactive compounds showed significant changes in the bands between 2786 to 3600 cm and 1022 to 1729 cm in the FT-IR spectrum. The highest antifungal activity against the microorganisms was observed in the Ekuanot extract at an extraction temperature of 65 °C. The test with bell peppers presented antifungal activity during five days of evaluation under normal environmental conditions without refrigeration, presenting ≤ 52% of the disease due to and .