Hepatoprotective Effect of Camel Thorn Polyphenols in Concanavalin A-Induced Hepatitis in Mice

• Published in: Chinese journal of integrative medicine 2024-12, Vol.30 (12), p.1090-1100
• Main Authors: El-Mahdy, Nageh Ahmed, El-Masry, Thanaa Ahmed, El-Tarahony, Ahmed Mahmoud, Alherz, Fatemah A., Osman, Enass Youssef
• Format: Article
• Language: English
• Subjects: Animals; Antioxidants - pharmacology; Apoptosis - drug effects; Camelus; CD4-Positive T-Lymphocytes - drug effects; Chemical and Drug Induced Liver Injury - drug therapy; Chemical and Drug Induced Liver Injury - pathology; Chemical and Drug Induced Liver Injury - prevention & control; Concanavalin A; Hepatitis - drug therapy; Hepatitis - pathology; Inflammation Mediators - metabolism; Liver - drug effects; Liver - pathology; Male; Medicine; Medicine & Public Health; Mice; Original Article; Oxidative Stress - drug effects; Plant Extracts - pharmacology; Plant Extracts - therapeutic use; Polyphenols - pharmacology; Protective Agents - pharmacology
• ISSN: 1672-0415; 1993-0402; 1993-0402
• DOI: 10.1007/s11655-024-3808-3

Objectives To explore the prophylactic and therapeutic effects of Alhagi maurorum ethanolic extract (AME) in concanavalin A (Con A)-induced hepatitis (CIH) as well as possible underlying mechanisms. Methods Polyphenols in AME were characterized using high performance liquid chromatography (HPLC). Swiss albino mice were divided into 4 groups. Normal group received intravenous phosphate-buffered saline (PBS); Con A group received 40 mg/kg intravenous Con A. Prophylaxis group administered 300 mg/(kg·d) AME orally for 5 days before Con A intervention. Treatment group received intravenous Con A then administered 300 mg/kg AME at 30 min and 3 h after Con A intervention. After 24 h of Con A injection, hepatic injury, oxidative stress, and inflammatory mediators were assessed. Histopathological examination and markers of apoptosis, inflammation, and CD4 + cell infiltration were also investigated. Results HPLC analysis revealed that AME contains abundant polyphenols with pharmacological constituents, such as ellagic acid, gallic acid, ferulic acid, methylgallate, and naringenin. AME alleviated Con A-induced hepatic injury, as manifested by a significant reduction in alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase ( P