Enhancing Performances of Enzyme/Metal–Organic Polyhedra Composites by Mixed-Protein Co-Immobilization

• Published in: ACS applied materials & interfaces 2024-10, Vol.16 (40), p.54423-54434
• Main Authors: Kanzaki, Yuri, Minami, Ryosuke, Ota, Koshiro, Adachi, Junya, Hori, Yuichiro, Ohtani, Ryo, Le Ouay, Benjamin, Ohba, Masaaki
• Format: Article
• Language: English
• Subjects: Functional Nanostructured Materials (including low-D carbon)
• ISSN: 1944-8244; 1944-8252; 1944-8252
• DOI: 10.1021/acsami.4c10146

Protein immobilization using water-soluble ionic metal–organic polyhedra (MOPs) acting as porous spacers has recently been demonstrated as a potent strategy for the preparation of biocatalysts. In this article, we describe a mixed-protein approach to achieve biocomposites with adjustable enzyme contents and excellent immobilization efficiencies, in a systematic and well-controlled manner. Self-assembly of either cationic or anionic MOPs with bovine serum albumin or egg white lysozyme combined with enzymes (alkaline phosphatase, laccase or cytochrome c) led to solid-state catalysts with a high retention of enzyme activity. Furthermore, for all these systems, the dilution of enzymes within the solid-state composite led to noticeably improved catalytic performances, with both higher specific activity and affinity for substrate.