Molecular analyses of the tubby-like protein gene family and their response to salt and high temperature in the foxtail millet (Setaria italica)

• Published in: Functional & integrative genomics 2024-10, Vol.24 (5), p.170, Article 170
• Main Authors: Jiang, Zhuanzhuan, Chen, Xiaoqi, Ruan, Lingling, Xu, Yan, Li, Ke
• Format: Article
• Language: English
• Subjects: Abiotic stress; Animal Genetics and Genomics; Biochemistry; Bioinformatics; Biomedical and Life Sciences; Cell Biology; Gene expression; Gene Expression Regulation, Plant; Heat resistance; High temperature; Hot Temperature; Life Sciences; Microbial Genetics and Genomics; Millet; Multigene Family; Phylogeny; Plant Genetics and Genomics; Plant Proteins - genetics; Plant Proteins - metabolism; Prokaryotes; Protein structure; Salt Stress; Setaria italica; Setaria Plant - genetics; Setaria Plant - metabolism; Stress, Physiological; Temperature; Tlp gene
• ISSN: 1438-793X; 1438-7948; 1438-7948
• DOI: 10.1007/s10142-024-01458-0

Tubby-like proteins (TLPs) are a group of proteins found in both eukaryotes and prokaryotes. They are significant in various physiological and biochemical processes, especially in plants’ response to abiotic stress. However, the role of TLP in foxtail millet ( Setaria italica ) remains unclear. The millet genome has 16 members of the TLP family with typical Tub domains, which can be sorted into five subgroups based on gene structure, motif, and protein domain distribution. SiTLPs were discovered to be predominantly located in the nucleus and also had extracellular distribution. The interspecific evolutionary analysis indicated that SiTLPs had a closer evolutionary relationship with monocots and were consistent with the morphological classification of foxtail millet. When subjected to salt stress, the abundance of SiTLP was affected, and qRT-PCR results showed that the expression levels of certain SiTLP members were induced by salt stress while others remained unresponsive. Except for SiTLP14 , all other SiTLP genes were up-regulated in response to high-temperature stress, implying a potentially crucial role for SiTLP in mitigating high-temperature-induced damage. This study provides valuable insights into understanding the functional significance of the TLP gene family in foxtail millet.