Genetically encoded epigenetic sensors for visualization of H3K9me3, H3K9ac and H3K4me1 histone modifications in living cells

Post-translational modifications of histones play a crucial role in chromatin structure maintenance and epigenetic regulation. The LiveMIEL (Live-cell Microscopic Imaging of Epigenetic Landscape) method represents a promising approach for tracking histone modifications. It involves visualization of...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2024-11, Vol.733, p.150715, Article 150715
Main Authors: Stepanov, Afanasii I., Putlyaeva, Lidia V., Besedovskaya, Zlata, Shuvaeva, Alexandra A., Karpenko, Nikita V., Rukh, Shah, Gorbachev, Dmitry A., Malyshevskaia, Kseniia K., Terskikh, Alexey V., Lukyanov, Konstantin A., Gurskaya, Nadya G.
Format: Article
Language:English
Subjects:
Epigenetics
Fluorescent proteins
Genetically encoded sensor
H3K4me1
H3K9ac
H3K9me3
Histone modification
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Summary:Post-translational modifications of histones play a crucial role in chromatin structure maintenance and epigenetic regulation. The LiveMIEL (Live-cell Microscopic Imaging of Epigenetic Landscape) method represents a promising approach for tracking histone modifications. It involves visualization of epigenetic modifications using genetically encoded fluorescent sensors and further analysis of the obtained intranuclear patterns by multiparametric image analysis. In this study, we designed three new red fluorescent sensors—MPP8-Red, AF9-Red and DPF3-Red—for live-cell visualization of patterns of H3K9me3, H3K8ac and H3K4me1, respectively. The observed fluorescent patterns were visually distinguishable, and LiveMIEL analysis clearly classified them into three corresponding groups. We propose that these sensors can be used for live-cell dynamic analysis of changes in organization of three epigenetic types of chromatin. •Genetically encoded fluorescent sensors were developed to visualize H3K9me3, H3K9ac, and H3K4me1 histone modifications in living cells.•Katushka is an excellent fluorescent core for epigenetic sensors due to its ability to dimerize, which is necessary for the effective visualization of histone modifications by HMRDs.•The LiveMIEL method enables dynamic analysis of changes in histone modification organization in living cells.
ISSN:0006-291X
1090-2104
1090-2104
DOI:10.1016/j.bbrc.2024.150715