Neuromodulator and neuropeptide sensors and probes for precise circuit interrogation in vivo

To determine how neuronal circuits encode and drive behavior, it is often necessary to measure and manipulate different aspects of neurochemical signaling in awake animals. Optogenetics and calcium sensors have paved the way for these types of studies, allowing for the perturbation and readout of sp...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 2024-09, Vol.385 (6716), p.eadn6671
Main Authors: Muir, J, Anguiano, M, Kim, C K
Format: Article
Language:English
Subjects:
Animals
Binding
Biological activity
Biology
Biosensing Techniques
Brain
Calcium - analysis
Calcium - metabolism
Chemical activity
Chemical engineering
Chemical sensors
Circuits
Decoupling
Drug delivery
Fluorescent indicators
G protein-coupled receptors
Genetic code
Genetic engineering
Genetics
Heterogeneity
In vivo methods and tests
Information processing
Infrared tracking
Integrators
Interrogation
Intracellular signalling
Kinetics
Learning
Machine learning
Nanosensors
Neural Pathways - physiology
Neuromodulation
Neurons - metabolism
Neurons - physiology
Neuropeptides
Neuropeptides - analysis
Neuroplasticity
Neurotransmitter Agents - analysis
Optics
Optogenetics
Probes
Protein engineering
Proteins
Reading Ability
Real time
Receptor mechanisms
Receptors
Recording
Sensitivity analysis
Sensors
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Summary:To determine how neuronal circuits encode and drive behavior, it is often necessary to measure and manipulate different aspects of neurochemical signaling in awake animals. Optogenetics and calcium sensors have paved the way for these types of studies, allowing for the perturbation and readout of spiking activity within genetically defined cell types. However, these methods lack the ability to further disentangle the roles of individual neuromodulator and neuropeptides on circuits and behavior. We review recent advances in chemical biology tools that enable precise spatiotemporal monitoring and control over individual neuroeffectors and their receptors in vivo. We also highlight discoveries enabled by such tools, revealing how these molecules signal across different timescales to drive learning, orchestrate behavioral changes, and modulate circuit activity.
ISSN:0036-8075
1095-9203
1095-9203
DOI:10.1126/science.adn6671