OsHRZ1 negatively regulates rice resistant to Magnaporthe oryzae infection by targeting OsVOZ2

• Published in: Transgenic research 2024-10, Vol.33 (5), p.489-501
• Main Authors: Sun, Jia-ying, Zhou, Zeng-ran, Wang, Yu-qi, Zhu, Dong-yu, Ma, Dian-rong
• Format: Article
• Language: English
• Subjects: Animal Genetics and Genomics; Ascomycota; Biomedical and Life Sciences; Biomedical Engineering/Biotechnology; Complementation; Crop yield; Disease resistance; Disease Resistance - genetics; Gene expression; Gene Expression Regulation, Plant; Genetic Engineering; Homeostasis; Humidity; Immune response; Infections; Inoculation; Iron; Iron - metabolism; Leaves; Life Sciences; Magnaporthe oryzae; Molecular Medicine; Mutants; Oryza - genetics; Oryza - microbiology; Pathogens; Plant Diseases - genetics; Plant Diseases - immunology; Plant Diseases - microbiology; Plant Genetics and Genomics; Plant Proteins - genetics; Plant Proteins - metabolism; Plant resistance; Plants; Plasmids; Rice; Rice blast; Transgenics; Ubiquitin; Ubiquitin-Protein Ligases - genetics; Ubiquitin-Protein Ligases - metabolism; Ubiquitination; Ubiquitination - genetics; Zinc finger proteins
• ISSN: 0962-8819; 1573-9368; 1573-9368
• DOI: 10.1007/s11248-024-00415-8

Rice blast disease caused by Magnaporthe oryzae significantly reduces yield production. Blast resistance is closely associated with iron (Fe) status, but the mechanistic basis linking iron status to immune function in rice remains largely unknown. Here, iron-binding haemerythrin RING ubiquitin ligases OsHRZ1 was confirmed to play key roles in iron-mediated rice blast resistance. The expression of OsHRZ1 was suppressed by M. oryzae inoculation and high iron treatment. Both mutants of OsHRZ1 enhanced rice resistance to M. oryzae . OsPR1a was up-regulated in OsHRZ1 mutants. Yeast two-hybrid, bimolecular fluorescence complementation, and Co-IP assay results indicated that OsHRZ1 interacts with Vascular Plant One Zinc Finger 2 (OsVOZ2) in the nucleus. Additionally, the vitro ubiquitination assay indicated that OsHRZ1 can ubiquitinate OsVOZ2 and mediate the degradation of OsVOZ2. The mutants of OsVOZ2 showed reduced resistance to M. oryzae and down-regulated the expression of OsPR1a . Yeast one-hybrid, EMSA, and dual-luciferase reporter assay results indicated that OsVOZ2 directly binds to the promoter of OsPR1a , activating its expression. In summary, OsHRZ1 plays an important role in rice disease resistance by mediated degradation of OsVOZ2 thus shaping PR gene expression dynamics in rice cells. This highlights an important link between iron signaling and rice pathogen defenses.