Fabrication of Thymol‐loaded Isabgol/Konjac Glucomannan‐based Microporous Scaffolds with Enriched Antioxidant and Antibacterial Properties for Skin Tissue Engineering Applications

• Published in: Chemistry, an Asian journal an Asian journal, 2024-12, Vol.19 (24), p.e202400839-n/a
• Main Authors: Sakthivel, Shruthi, Thangavel, Ponrasu, Saravanakumar, Iniyan, Muthuvijayan, Vignesh
• Format: Article
• Language: English
• Subjects: Animals; Anti-Bacterial Agents - chemical synthesis; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Antibacterial; Antioxidant; Antioxidants; Antioxidants - chemical synthesis; Antioxidants - chemistry; Antioxidants - pharmacology; Biocompatibility; Biocompatible Materials - chemical synthesis; Biocompatible Materials - chemistry; Biocompatible Materials - pharmacology; Biomedical materials; Biopolymers; Cell Line; Cell Proliferation - drug effects; Damage prevention; Drug delivery; Escherichia coli - drug effects; Fibroblasts - drug effects; Freezing; Isabgol; Konjac glucomannan; Mannans - chemistry; Mannans - pharmacology; Mice; Microbial Sensitivity Tests; Polymers; Porosity; Regeneration (physiology); Scaffolds; Skin - drug effects; Staphylococcus aureus - drug effects; Thymol; Thymol - chemistry; Thymol - pharmacology; Tissue Engineering; Tissue Scaffolds - chemistry
• ISSN: 1861-4728; 1861-471X; 1861-471X
• DOI: 10.1002/asia.202400839

An antioxidant, antibacterial, and biocompatible biomaterial is essential to repair skin wounds effectively. Here, we have employed two natural biopolymers, isabgol (ISAB) and konjac glucomannan (KGM), to prepare microporous scaffolds by freezing and lyophilization. The scaffolds are loaded with thymol (THY) to impart potent antioxidant and antibacterial activities. The physicochemical properties of the ISAB+KGM+THY scaffold, like porosity (41.8±2.4 %), swelling, and biodegradation, were optimal for tissue regeneration application. Compared to the control, ISAB+KGM+THY scaffolds promote attachment, migration, and proliferation of L929 fibroblasts. The antioxidant activity of the ISAB+KGM+THY scaffold was significantly improved after loading THY. This would protect the tissues from oxidative damage. The antibacterial activity of the ISAB+KGM+THY scaffold was significantly higher than that of the control, which would help prevent bacterial infection. The vascularization ability of the ISAB+KGM scaffold was not altered by incorporating THY in the ISAB+KGM scaffold. Therefore, a strong antioxidant, antibacterial, and biocompatible nature of the ISAB+KGM+THY scaffold could be useful for various biomedical applications. Thymol‐loaded ISAB+KGM composite biocompatible scaffold shows antioxidant and antibacterial properties.