Enhancement of Bioactive Compounds and Survival of Lactobacillus acidophilus Grown in the Omega-6, -7 Riched Cyanobacteria Spirulina platensis

• Published in: Current microbiology 2024-11, Vol.81 (11), p.380, Article 380
• Main Authors: Hoang, Phuong Ha, Nguyen, Minh T., Ngo, Hoai Thu T., Chu, Nhat Huy, Ha, Phuong Thu, Bui, Huong Giang, To, Linh Hang
• Format: Article
• Language: English
• Subjects: Algae; Anti-Bacterial Agents - pharmacology; Antibacterial activity; Antiinfectives and antibacterials; Aquaculture; Bioactive compounds; Biological activity; Biomedical and Life Sciences; Biotechnology; Culture Media - chemistry; Culture Media - metabolism; Cyanobacteria; Fatty Acids, Omega-6 - metabolism; Fatty Acids, Unsaturated - metabolism; Fermentation; Immune system; Lactic acid; Lactic Acid - metabolism; Lactobacilli; Lactobacillus acidophilus; Lactobacillus acidophilus - growth & development; Lactobacillus acidophilus - metabolism; Life Sciences; Microbial Viability - drug effects; Microbiology; Polyunsaturated fatty acids; Probiotics; Probiotics - metabolism; Sodium chloride; Spirulina - chemistry; Spirulina - growth & development; Spirulina - metabolism; Spirulina platensis; Survival
• ISSN: 0343-8651; 1432-0991; 1432-0991
• DOI: 10.1007/s00284-024-03865-9

Lactobacillus acidophilus is a probiotic commonly used in aquaculture to enhance the growth and immune system of aquatic species through the synthesis of various enzymes, and antimicrobial compounds like lactic acid. Traditional method of growing L. acidophilus involes using the De Man–Rogosa–Sharpe (MRS) medium. However, L. acidophilus belongs to a non-spore forming group, which make it vulnerable to stress conditions, especially during the usage process. Therefore, the present study aimed to improve the survival rate, antibacterial activity, and enrich the polyunsaturated fatty acids (PUFAs) content of L. acidophilus LB when cultured in an algae-supplemented medium, thus increasing its benefits in aquaculture applications. Using different algae biomass species as an alternative to MRS medium for the growth of L. acidophilus LB, the results showed that Spirulina platensis promoted the highest density of L. acidophilus LB. When grown in ( S. platensis  + glucose) medium, L. acidophilus LB produced the highest lactic acid concentration of 18.24 ± 2.43 mg/mL and survived in extreme conditions such as 4% NaCl, pH 1.0–2.0, and 50 ºC, and inhibited 99.82 ± 0.24% of Vibrio parahaemolyticus population after 2 days of treatment. Additionally, it was observed that the PUFAs content, specifically omega-6, and -7, also increased in the fermentation mixture as compared to the control sample. These findings highlighted the potential of utilizing the cyanobacteria S. platensis as an alternative, eco-friendly growth substance for L. acidophilus LB to enhance its bioactivity and viability under extreme conditions. Graphical Abstract