Therapeutic potential of Shaoyao Gancao Decoction in mitigating anti‐tuberculosis drug‐induced liver injury through Nrf‐2/HO‐1/NF‐κB signaling

• Published in: Biomedical chromatography 2024-12, Vol.38 (12), p.e6016-n/a
• Main Authors: Zhang, Huan, Ma, Lihua, Li, Sisi, Ding, Qiaoyan, Zhang, Yu, Zhou, Ming
• Format: Article
• Language: English
• Subjects: Animals; Antitubercular Agents; anti‐tuberculosis drugs; Chemical and Drug Induced Liver Injury - drug therapy; Chemical and Drug Induced Liver Injury - metabolism; Chromatography, High Pressure Liquid - methods; Disease Models, Animal; Drugs, Chinese Herbal - pharmacology; Heme Oxygenase (Decyclizing) - metabolism; Liver - drug effects; Liver - metabolism; liver injury; Male; network pharmacology analysis; NF-E2-Related Factor 2 - metabolism; NF-kappa B - metabolism; Nrf‐2/HO‐1/NF‐κB signaling pathway; Oxidative Stress - drug effects; Rats; Rats, Sprague-Dawley; Shaoyao Gancao Decoction; Signal Transduction - drug effects; Tandem Mass Spectrometry - methods; tuberculosis
• ISSN: 0269-3879; 1099-0801; 1099-0801
• DOI: 10.1002/bmc.6016

Tuberculosis (TB) is a persistent global health issue, evidenced by an increasing number of cases. Although anti‐TB drugs have proven efficacy, they are often associated with severe liver injury (ATB‐DILI). The objective of this research was to uncover the mechanisms through which Shaoyao Gancao Decoction (SGD) mitigates ATB‐DILI, emphasizing the role of the Nrf‐2/HO‐1/NF‐κB signaling pathway. We prepared SGD granules and subjected them to HPLC‐MS/MS for analysis. An ATB‐DILI rat model was then developed and administered SGD. We evaluated liver injury markers, the extent of oxidative stress, inflammation, and the principal proteins involved in the Nrf‐2/HO‐1/NF‐κB pathway. Additionally, network pharmacology techniques were utilized to discern potential SGD targets and their associated pathways. Administering SGD had a notable effect in counteracting the elevation of liver injury markers and pathological alterations induced by ATB‐DILI. Moreover, there was a marked reduction in oxidative stress and inflammation in the treated rats. We identified 12 active compounds in SGD, with 88 shared targets between SGD and ATB‐DILI. Subsequent KEGG analysis brought attention to pathways like MAPK, NF‐κB, and IL‐17 signaling. Our findings pave the way for more in‐depth studies into the application of SGD in treating drug‐induced liver injuries.