Electrophysiological and Hemodynamic Mechanisms Underlying Load Modulations in Visuospatial Working Memory: A Functional Near-Infrared Spectroscopy (fNIRS) and Electroencephalogram (EEG) Study

• Published in: Behavioral neuroscience 2024-10, Vol.138 (5), p.331-341
• Main Authors: Zarantonello, Lisa, Brigadoi, Sabrina, Schiff, Sami, Bisiacchi, Patrizia Silvia, Cutini, Simone, Montagnese, Sara, Amodio, Piero
• Format: Article
• Language: English
• Subjects: Adult; Anoxia; Blood Flow; Brain - diagnostic imaging; Brain - physiology; Cortex (frontal); EEG; Electroencephalography; Electroencephalography - methods; Female; Frontal Lobe; Hemodynamics; Hemodynamics - physiology; Hemoglobin; Human; Humans; Infrared spectroscopy; Male; Memory, Short-Term - physiology; Mental task performance; Reaction Time - physiology; Short Term Memory; Spatial memory; Spectroscopy; Spectroscopy, Near-Infrared - methods; Spectrum analysis; Visual evoked potentials; Visual Perception - physiology; Visuospatial Ability; Young Adult
• ISSN: 0735-7044; 1939-0084; 1939-0084
• DOI: 10.1037/bne0000604

The n-back task has been widely used to study working memory. Previous studies investigating the electrophysiological (electroencephalogram [EEG]) and hemodynamic correlates (functional near-infrared spectroscopy [fNIRS]) of the n-back task have been generally based on verbal stimuli and only investigated EEG frequency bands. We simultaneously acquired the EEG and fNIRS in 35 participants (16 males; age = 26.4 ± 4.3 years; educational attainment = 18 ± 2 years) during a visuospatial n-back task. The task encompassed a control condition and a low (requiring to recall one previous stimulus) and a high (requiring to recall two previous stimuli) working memory load experimental conditions. Accuracy decreased and reaction times slowed in the high compared to both low load and control conditions. Regarding EEG, P3a showed higher amplitude in the experimental conditions compared to the control one, and P3b exhibited higher amplitude in the low compared to the high load condition. Regarding fNIRS, the high load condition showed higher deoxygenated hemoglobin compared to the control one. Moreover, the central frontopolar cortex showed higher activation compared with the left frontal cortex. Our study showed that working memory load during a visuospatial n-back task influenced behavioral and electrophysiological indices. Even if the load effect was only observed for deoxygenated hemoglobin on hemodynamic data, this was in line with previous studies and coherent with its electrophysiological correlates. Thus, our study confirms that EEG and fNIRS can be successfully used in multimodal acquisitions, but also highlights that future studies are needed to develop a novel version of the task.