The S-nitrosylation of monodehydroascorbate reductase positively regulated the low temperature tolerance of mini Chinese cabbage

• Published in: International journal of biological macromolecules 2024-11, Vol.281 (Pt 1), p.136047, Article 136047
• Main Authors: Gao, Xueqin, Ma, Jizhong, Wang, Guangzheng, Huang, Shuchao, Wu, Xuetong, Hu, Linli, Yu, Jihua
• Format: Article
• Language: English
• Subjects: Antioxidants - metabolism; Brassica - genetics; Brassica - metabolism; Brassinosteroid; Brassinosteroids - metabolism; Cold Temperature; Gene Expression Regulation, Plant; Low temperature; Mini Chinese cabbage; Monodehydroascorbate reductase; NADH, NADPH Oxidoreductases - genetics; NADH, NADPH Oxidoreductases - metabolism; Nitric Oxide - metabolism; Plant Proteins - genetics; Plant Proteins - metabolism; S-Nitrosothiols - metabolism; S-nitrosylation
• ISSN: 0141-8130; 1879-0003; 1879-0003
• DOI: 10.1016/j.ijbiomac.2024.136047

One of the main environmental stresses that considerably reduced vegetable yields are low temperature stress. Brassinosteroids (BRs) is essential for controlling a number of physiological functions. Protein S-nitrosylation is thought to be a crucial process in plants that use NO to carry out their biological functions. The exact process by which the mini Chinese cabbage responded to low temperature stress through BR-mediated S-nitrosylation modification of the monodehydroascorbate reductase (MDHAR) is still unknown. BR significantly increased the S-nitrosoylation level and antioxidant capacity at low temperature. One noteworthy development was the in vitroS-nitrosylation of the MDHAR protein. The overexpressed lines exhibited considerably high nitric oxide (NO) and S-nitrosothiol (SNO) contents at low temperature compared to the WT lines. Treatment of the WT and OE-BrMDHAR lines with BR at low temperature increased the antioxidant capacity. According to the biotin signaling, BR considerably enhanced the silenced lines total S-nitrosylation level in vivo at low temperature. Furthermore, BrMDHAR, BrAAO, and BrAPX gene transcript levels were dramatically up-regulated by BR, which in turn reduced the H2O2 content in the silenced lines. These findings demonstrated that the S-nitrosylation of MDHAR was essential to the improvement of BR on low-temperature tolerance in the mini Chinese cabbage. •BR positively regulated the endogenous S-nitrosylation level at low temperature.•MDHAR protein was modified by S-nitrosylation in vitro.•OE-BrMDHAR increased the S-nitrosylation protein abundance of MDHAR protein at low temperature.•Exogenous BR increased the antioxidant capacity of TRV2-BrMDHAR line at low temperature.