Strontium-containing mineralized phospholipid coatings improve osseointegration in osteoporotic rats

• Published in: Journal of biomedical materials research. Part A 2025-01, Vol.113 (1), p.e37782
• Main Authors: Fernandes E Oliveira, Hiskell Francine, Addison, Owen, Yogui, Fernanda C, Cruz, Marcos Antonio Eufrásio, Nogueira, Lucas Fabricio Bahia, Okamoto, Roberta, Souza, Ana Carla Gonçales, Ramos, Ana Paula, Verri, Fellippo Ramos
• Format: Article
• Language: English
• Subjects: Animals; Bone Screws; Coated Materials, Biocompatible - chemistry; Coated Materials, Biocompatible - pharmacology; Durapatite - chemistry; Durapatite - pharmacology; Female; Osseointegration - drug effects; Osteoporosis - drug therapy; Phospholipids - chemistry; Rats; Rats, Sprague-Dawley; Strontium - chemistry; Strontium - pharmacology; Surface Properties; Titanium - chemistry; Titanium - pharmacology
• ISSN: 1549-3296; 1552-4965; 1552-4965
• DOI: 10.1002/jbm.a.37782

Surface treatments play an important role in enhancing the osseointegration of Titanium (Ti) and its alloys. This study introduces a method employing biomimetic hydroxyapatite (Hap) deposition guided by molecularly organized phospholipids, affixed to the metal implant surface. Using the Langmuir-Blodgett technique, phospholipids were deposited onto Ti-screws by using CaCl or CaCl /SrCl aqueous solution in the subphase of a Langmuir trough in the target proportion (i.e. 10 and 90 mol% of Sr in relation of Ca ) followed by immersion in phosphate buffer and in supersaturated simulated body fluid. Coating composition and morphology were evaluated using infrared spectroscopy and scanning electron microscopy, respectively, while contact angle measurements assessed coating wettability and surface energy. Randomized screws were then implanted into the tibias of healthy and osteoporotic female rats (G1: Control-Machined, G2: Hap, G3: HapSr10, G4: HapSr90). Osseointegration, assessed 60 days post-implantation, included reverse torque, fluorochrome area, bone tissue-screw contact area, and linear extent of bone-screw contact. Results, grouped by surface treatment (Machined, Hap, HapSr10, HapSr90), revealed that the deposition of Hap, HapSr10, and HapSr90 resulted in thin and rough coatings composed of hydroxyapatite (Hap) on the screw surface with nanoscale pores. The coatings resulted in increased wettability and surface energy of Ti surfaces. The minerals are chemically similar to natural bone apatite as revealed by FTIR analysis. In vivo analyses indicated higher torque values for strontium-containing surfaces in the osteoporotic group (p = 0.02) and, in the control group superior torque for screw removal on the Hap surface (p = 0.023). Hydroxyapatite-treated surfaces enhance morphology, composition, and reactivity, promoting screw osseointegration in healthy and osteoporotic female rats. The incorporation of strontium into the mineral phase has been proposed to not only stimulate osteoblast activity but also reduce osteoclastic resorption, which may explain the improved outcomes observed here in experimental osteoporotic conditions.