Development of wheat-tetraploid Thinopyrum elongatum 4EL small fragment translocation lines with stripe rust resistance gene Yr4EL

• Published in: Theoretical and applied genetics 2024-10, Vol.137 (10), p.246, Article 246
• Main Authors: Gong, Biran, Gao, Jing, Xie, Yangqiu, Zhang, Hao, Zhu, Wei, Xu, Lili, Cheng, Yiran, Wang, Yi, Zeng, Jian, Fan, Xing, Sha, Lina, Zhang, Haiqin, Zhou, Yonghong, Wu, Dandan, Li, Yinghui, Kang, Houyang
• Format: Article
• Language: English
• Subjects: Agriculture; Basidiomycota - pathogenicity; Biochemistry; Biomedical and Life Sciences; Biotechnology; Chromosome Mapping; Chromosomes; Chromosomes, Plant - genetics; Cultivars; Diploids; Disease resistance; Disease Resistance - genetics; Fluorescence in situ hybridization; Genes; Genes, Plant; Genetic Markers; Genomes; Genomic in situ hybridization; Germplasm; In Situ Hybridization, Fluorescence; Laboratories; Life Sciences; Marker-assisted selection; Original Article; Plant Biochemistry; Plant Breeding; Plant Breeding/Biotechnology; Plant Diseases - genetics; Plant Diseases - microbiology; Plant Genetics and Genomics; Poaceae - genetics; Poaceae - microbiology; Puccinia - pathogenicity; Single-nucleotide polymorphism; Stripe rust; Tetraploidy; Thinopyrum elongatum; Translocation, Genetic; Triticum - genetics; Triticum - microbiology; Virulence
• ISSN: 0040-5752; 1432-2242; 1432-2242
• DOI: 10.1007/s00122-024-04756-0

Key message Two small fragment translocation lines (T4DS·4DL-4EL and T5AS·5AL-4EL) showed high resistance to stripe rust and resistance gene Yr4EL was localized to an about 35 Mb region at the end of chr arm 4EL. Stripe rust, caused by the fungus Puccinia striiformis f. sp. tritici , is a devastating wheat disease worldwide. Deployment of disease resistance ( R ) genes in wheat cultivars is the most effective way to control the disease. Previously, the all-stage stripe rust R gene Yr4EL from tetraploid Thinopyrum elongatum was introduced into common wheat as 4E(4D) substitution and T4DS·4EL translocation lines. To further map and utilize Yr4EL , Chinese Spring (CS) mutant pairing homoeologous gene ph1b was used in crossing to induce recombination between chromosome (chr) 4EL and wheat chromosomes. Two small fragment translocation lines T4DS·4DL-4EL and T5AS·5AL-4EL with Yr4EL resistance were selected using molecular markers and confirmed by genomic in situ hybridization (GISH), fluorescence in situ hybridization (FISH), and Wheat 660 K SNP array analyses. We mapped Yr4EL to an about 35 Mb region at the end of chr 4EL, corresponding to 577.76–612.97 Mb based on the diploid Th. elongatum reference genome. In addition, two competitive allele-specific PCR (KASP) markers co-segregating with Yr4EL were developed to facilitate molecular marker-assisted selection in breeding. The T4DS·4DL-4EL lines were crossed and backcrossed with wheat cultivars SM482 and CM42, and the resulting pre-breeding lines showed high stripe rust resistance and potential for wheat breeding with good agronomic traits. These lines represent new germplasm for wheat stripe rust resistance breeding, as well as providing a solid foundation for Yr4EL fine mapping and cloning.