Unveiling placental development in circadian rhythm-disrupted mice: A photo-acoustic imaging study on unstained tissue

• Published in: Placenta (Eastbourne) 2024-12, Vol.158, p.57-61
• Main Authors: Cizmeciyan, M.N., Bektas, N.I., Derin, N., Denizaltı, T., Khoshzaban, A., Unlu, M.B., Celik-Ozenci, C.
• Format: Article
• Language: English
• Subjects: Animals; Circadian rhythm; Circadian Rhythm - physiology; Female; Fetal Development - physiology; Histology; Mice; Mice, Inbred C57BL; Microscopy, Acoustic - methods; Photo-acoustic microscopy; Photoacoustic Techniques - methods; placenta; Placenta - diagnostic imaging; Placentation - physiology; Pregnancy
• ISSN: 0143-4004; 1532-3102; 1532-3102
• DOI: 10.1016/j.placenta.2024.10.001

Circadian rhythm disruption has garnered significant attention for its adverse effects on human health, particularly in reproductive medicine and fetal well-being. Assessing pregnancy health often relies on diagnostic markers such as the labyrinth zone (LZ) proportion within the placenta. This study aimed to investigate the impact of disrupted circadian rhythms on placental health and fetal development using animal models. Employing unstained photo-acoustic microscopy (PAM) and hematoxylin and eosin (HE)-stained images, we found them mutually reinforcing. Our images revealed the role of maternal circadian rhythm disrupted group (MCRD) on the LZ and fetus weight: a decrease in LZ area from 5.01 (4.25) mm2 HE (PAM) to 3.58 (2.62) mm2 HE (PAM) on day 16 and 6.48 (5.16) mm2 HE (PAM) to 4.61 (3.03) mm2 HE (PAM) on day 18, resulting in 0.71 times lower fetus weights. We have discriminated a decrease in the mean LZ to placenta area ratio from 64 % to 47 % on day 18 in mice with disrupted circadian rhythms with PAM. The study highlights the negative influence of circadian rhythm disruption on placental development and fetal well-being. Reduced LZ area and fetal weights in the MCRD group suggest compromised placental function under disrupted circadian rhythms. PAM imaging proved to be an efficient technique for assessing placental development, offering advantages over traditional staining methods. These findings contribute to understanding the underlying mechanisms of circadian disruption on reproductive health and fetal development. Further research is needed to explore interventions to mitigate these effects and improve pregnancy outcomes. •PAM images are complementary to the HE-stained versions without staining.•PAM visualized subtle alterations caused by circadian rhythm-disruption on placenta.•Circadian rhythm-disrupted mice had smaller labyrinth zone, and placenta.•Circadian rhythm-disrupted mice delivered 0.71 times lighter fetuses by weight.•Placental compensatory effort was observed going from 16 to 18 days in pregnancy.