The effect of male factors on embryo morphokinetics: a retrospective analysis of 2726 blastocysts

Male infertility may influence fertilization rates, embryo morphology, and implantation rates in in vitro fertilization (IVF) cycles. Oocyte competence plays a major role in embryo development, but there is a limited understanding of the connection between sperm quality, embryo development, and morp...

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Published in:Journal of assisted reproduction and genetics 2024-10
Main Authors: Pellegrini, Livia, Gatti, Simona, Navarro, Nuria, Hervas, Irene, Marcos, Meseguer, Viviana, Vásquez, Toschi, Marco, Galliano, Daniela, Cozzolino, Mauro
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container_title Journal of assisted reproduction and genetics
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creator Pellegrini, Livia
Gatti, Simona
Navarro, Nuria
Hervas, Irene
Marcos, Meseguer
Viviana, Vásquez
Toschi, Marco
Galliano, Daniela
Cozzolino, Mauro
description Male infertility may influence fertilization rates, embryo morphology, and implantation rates in in vitro fertilization (IVF) cycles. Oocyte competence plays a major role in embryo development, but there is a limited understanding of the connection between sperm quality, embryo development, and morphokinetic parameters using donor oocytes. The study evaluated if sperm quality may influence the morphokinetic parameters in IVF cycles. A retrospective multicentric observational cohort study included 747 ICSI cycles using donor oocytes with fresh or frozen sperm. Embryos were cultured in time-lapse incubators until the blastocyst stage. The population was divided into three groups according to sperm concentration, as control group (> 16 mill/mL), severe oligospermia (0-5 mill/mL), and moderate oligospermia group (5-16 mill/mL). Morphokinetic analysis showed no difference in the time from the 2-cell to 6-cell stage of embryo development. A significant difference was observed on day 3 of embryo development, specifically at the 7-cell stage (t7), severe oligospermia 53.37 ± 9.81, moderate oligospermia 56.95 ± 9.78, and control 55.1 ± 8.85 h post-insemination (hpi) (p = 0.024), and 8-cell stage (t8), severe oligospermia 55.41 ± 10.83, moderate oligospermia 61.86 ± 12.38 hpi (p 
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title The effect of male factors on embryo morphokinetics: a retrospective analysis of 2726 blastocysts
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