High-fat and high-sugar diets induce rapid adaptations of fat storage in the house fly Musca domestica L

• Published in: Journal of evolutionary biology 2024-11, Vol.37 (11), p.1368-1377
• Main Authors: Boatta, Francesco, van Hal, Jurgen, Beukeboom, Leo W, Ellers, Jacintha
• Format: Article
• Language: English
• Subjects: Adaptation, Physiological; Animals; Diet; Diet, High-Fat; Dietary Fats; Dietary Sugars - administration & dosage; Houseflies - growth & development; Larva - growth & development
• ISSN: 1420-9101; 1420-9101
• DOI: 10.1093/jeb/voae122

Dietary change can be a strong evolutionary force and lead to rapid adaptation in organisms. High-fat and high-sugar diets can challenge key metabolic pathways, negatively affecting other life history traits and inducing pathologies such as obesity and diabetes. In this study, we use experimental evolution to investigate the plastic and evolutionary responses to nutritionally unbalanced diets. We reared replicated lines of larvae of the housefly Musca domestica on a fat-enriched (FAT), a sugar-enriched (SUG), and a control (CTRL) diet for thirteen generations. We measured development time in each generation and larval growth and fat accumulation in generations 1, 7, and 13. Subsequently, all lines were reared for one generation on the control diet to detect any plastic and evolutionary changes. In the first generation, time to pupation decreased on a fat-rich diet and increased on a sugar-rich diet. The fat-rich diet increased fat accumulation and, to a lesser extent, the dry weight of the larvae. Multigenerational exposure to unbalanced diets caused compensatory changes in development time, dry weight, and absolute and relative fat content, although pattern and timing depended on diet and trait. When put back on a control diet, many of the changes induced by the unbalanced diets disappeared, indicating that the diet has large plastic effects. Nevertheless, fat-evolved lines still grew significantly larger than the sugar-evolved lines, and sugar-evolved lines had consistently lower fat content. This can be an effect of parental diet or an evolutionary change in nutrient metabolism as a consequence of multigenerational exposure to unbalanced diets.