Abrasive challenge effects on enamel and dentin from irradiated human teeth: an in vitro study

• Published in: Radiation and environmental biophysics 2024-11, Vol.63 (4), p.573-582
• Main Authors: Arid, Juliana, Palma-Dibb, Regina Guenka, Molena, Kelly Fernanda, Faraoni, Juliana Jendiroba, Fillus, Thaís Marília, Tanimoto, Helio Massaiochi, Ranieri, Ana Laura Polizel, Paula-Silva, Francisco Wanderley Garcia de, Nelson-Filho, Paulo, de Macedo, Leandro Dorigan, Queiroz, Alexandra Mussolino de
• Format: Article
• Language: English
• Subjects: Abrasive wear; Aged; Biological and Medical Physics; Biophysics; Chemoradiotherapy; Chemotherapy; Dental enamel; Dental Enamel - radiation effects; Dentin; Dentin - radiation effects; Ecosystems; Effects of Radiation/Radiation Protection; Environmental Physics; Ethical standards; Exposure; Female; Head & neck cancer; Head and Neck Neoplasms - radiotherapy; Humans; Ionizing radiation; Male; Middle Aged; Monitoring/Environmental Analysis; Normality; Physics; Physics and Astronomy; Radiation therapy; Research ethics; Statistical analysis; Surface Properties; Surface roughness; Teeth
• ISSN: 0301-634X; 1432-2099; 1432-2099
• DOI: 10.1007/s00411-024-01094-z

This study aimed to evaluate the effects of radiotherapy (RT) and chemoradiotherapy (CRT) on the wear and surface roughness of in vitro irradiated human enamel and dentin subjected to abrasive challenge. Enamel and dentin specimens (n = 42) were prepared from teeth donated by healthy patients and those with head and neck cancer who had received radiotherapy (RT) or chemoradiotherapy (CRT). The specimens were categorized into three groups: control, RT, and CRT (n = 14 per group for both enamel and dentin). These samples were subjected to an in vitro abrasive experiment using a brushing machine, followed by wear and surface roughness assessments with a confocal laser scanning microscope conducted before and after the abrasive challenge, considering both exposed and non-exposed areas. Statistical analysis used Shapiro–Wilk tests for normality, Wilcoxon tests for comparing two means, and Kruskal–Wallis tests. A significance level of 5% was adopted. In enamel specimens, wear profile values ​​of CRT and RT groups were not different from the control ( p  > 0.05). The RT group presents lower step values than the CRT and control groups ( p   0.05). In dentin specimens, no significant difference in wear profile and step was observed in all groups ( p  > 0.05). However, CRT and RT groups present higher values in final surface roughness ( p