In vitro exposure of porcine spermatozoa to methylparaben, and propylparaben, alone or in combination adversely affects sperm quality

• Published in: Journal of applied toxicology 2024-10, Vol.44 (10), p.1540-1554
• Main Authors: Barraza, J., Cleofas, P., Villamil, S., García, M., López, A., Casas, E., Salazar, Z., Pichardo, F., Barajas‐Salinas, A., Núñez‐Macías, E., Ramírez, Y., Bonilla, E., Bahena, I., Ortíz‐Muñíz, R., Cortés‐Barberena, E., Betancourt, M., Casillas, F.
• Format: Article
• Language: English
• Subjects: Acrosome - drug effects; Animals; Cell Survival - drug effects; Deoxyribonucleic acid; DNA; DNA damage; DNA fragmentation; DNA Fragmentation - drug effects; Dose-Response Relationship, Drug; Exposure; Food industry; Infertility; Integrity; Male; methylparaben; mixture; Mixtures; Motility; Parabens - toxicity; Parameters; Physiological effects; porcine; Preservatives; Preservatives, Pharmaceutical - toxicity; Propyl paraben; propylparaben; Sperm; Sperm Motility - drug effects; sperm quality; Spermatozoa; Spermatozoa - drug effects; Swine; Toxicity; Viability
• ISSN: 0260-437X; 1099-1263; 1099-1263
• DOI: 10.1002/jat.4650

Parabens (PBs) are widely used in the cosmetic, pharmaceutical, and food industries as preservatives of products. Because of its great use, humans and other organisms are highly exposed daily. However, little is known about the effect of PBs on male infertility. Therefore, the aim of the present study was to evaluate the effect of methylparaben (MePB) and propylparaben (PrPB), alone or in combination, on the physiological characteristics of pig in vitro exposed sperm to different concentrations (0, 200, 500, and 700 μM) for viability, motility, and acrosome integrity evaluation and (0, 200, 500, 700, 1000, and 2000 μM) for DNA fragmentation index evaluation, after 4 h of exposure. The results showed that sperm viability decreased after exposure to MePB from the concentration of 500 μM. In the PrPB and mixture groups, viability decreased at all concentrations except for the control. The decrease in viability of sperm exposed to PrPB was greater than that of the mixture and MePB groups. Sperm motility decreased in all the experimental groups exposed to PBs, at all concentrations, except for the control group. Acrosome integrity was not decreased in the MePB group; however, in the PrPB group, it decreased at a concentration of 200 μM and in the mixture at 500 μM. All groups exhibited DNA damage at different concentrations, except for the control group. Additionally, the effect of PBs on sperm quality was concentration‐dependent. The results demonstrated that MePB and PrPB alone or in combination can have adverse effects on sperm quality parameters. MePB had lower toxicity than did both PrPB and the mixture. The mixture did not have an additive effect on any of the parameters evaluated. This could partially explain the link between PB exposure and infertility. Sperm viability decreased after exposure to MePB from the concentration of 500 μM. In the PrPB and mixture groups, viability decreased at all concentrations. Sperm motility decreased in all the experimental groups exposed to PBs at all concentrations. Acrosome integrity was not decreased in the MePB group; however, in the PrPB group, it decreased at a concentration of 200 μM and in the mixture at 500 μM. All groups exhibited DNA damage at different concentrations.