Determination of lawsone in henna powders by high performance thin layer chromatography

The lawsone content has been evaluated quantitatively in eight commercial henna powders and two collected henna leaves. The phenolic, chloroform-soluble fraction of the majority of the examined samples showed the presence of lawsone and two other pigments. Here we aimed to optimize high performance...

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Bibliographic Details
Published in:Journal of separation science 2007-12, Vol.30 (18), p.3311-3315
Main Authors: El-Shaer, Nagwa S, Badr, Jihan M, Aboul-Ela, Maha A, Gohar, Yousry M
Format: Article
Language:English
Subjects:
Analytical chemistry
Animals
Applied sciences
Biological and medical sciences
Chemical industry and chemicals
Chemistry
Chromatographic methods and physical methods associated with chromatography
Chromatography, Thin Layer - methods
Cosmetics, toiletries
Domestic and cosmetic products toxicology
Exact sciences and technology
Henna powders
HPTLC
In vivo toxicity studies
Lawsone
Lawsonia alba
Lawsonia Plant - chemistry
Male
Medical sciences
Molecular Structure
Naphthoquinones - analysis
Naphthoquinones - chemistry
Other chromatographic methods
Powders - administration & dosage
Powders - chemistry
Powders - toxicity
Rats
Sensitivity and Specificity
Toxicity Tests
Toxicology
Washing products. Cosmetics and toiletries. Perfumes
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Summary:The lawsone content has been evaluated quantitatively in eight commercial henna powders and two collected henna leaves. The phenolic, chloroform-soluble fraction of the majority of the examined samples showed the presence of lawsone and two other pigments. Here we aimed to optimize high performance thin layer chromatography for the determination of lawsone. Upon using the optimized method the examined samples showed considerable variation in lawsone concentration ranging from 0.004 up to 0.608 wt%, indicating that some samples were almost devoid of lawsone. Some of these products were subjected to preliminary in vivo toxicity studies.
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.200700223