Micro PIXE mapping proves a differential distribution and concentration of trace elements in fungal structures of Rhizophagus intraradices

• Published in: Fungal biology 2024-11, Vol.128 (7), p.2089-2093
• Main Authors: Benavidez, M.E., de la Fournière, E.M., Colombo, R.P., Silvani, V.A., Debray, M.E., Scotti, A., Godeas, A.M.
• Format: Article
• Language: English
• Subjects: Extraradical mycelium; Glomeromycota - chemistry; Micro PIXE; Mycelium - chemistry; Mycelium - growth & development; Mycelium - metabolism; Mycorrhizae - chemistry; Mycorrhizae - metabolism; Plant Roots - microbiology; R. intraradices; Rhizosphere; Soil Microbiology; Spectrometry, X-Ray Emission; Spores; Spores, Fungal - chemistry; Spores, Fungal - growth & development; Trace elements; Trace Elements - analysis; Trace Elements - metabolism
• ISSN: 1878-6146
• DOI: 10.1016/j.funbio.2024.08.008

Arbuscular mycorrhizal (AM) fungi can sequester different potentially toxic elements, such as trace elements (TEs), within their structures to alleviate the toxicity for its host plant and themselves. To elucidate the role of AM fungi in TEs immobilization in the rhizosphere of host plants, it is important to know the TEs distribution in AM fungal structures. In the present study, we investigated the distribution and concentration of TEs within extraradical spores and mycelium of the AM fungus Rhizophagus intraradices, collected from the rhizosphere of Senecio bonariensis plants grown in a soil polluted with multiple TEs, by using Particle-Induced X-ray Emission with a micro-focused beam (micro PIXE). This technique enabled the simultaneous micrometric mapping of elements in a sample. The calculated values were compared with those in the polluted substrate, measured by the Wavelength Dispersive X-ray Fluorescence technique. The highest concentrations of Fe, P, Ti, Mn, Cr, Cu and Zn were found in AM fungal spores, where they were accumulated, while extraradical mycelium was enriched in Cu. Finally, we demonstrated that AM fungi can simultaneously accumulate high amounts of different TEs in their structures, thus reducing the toxicity of these elements to its host plant. •Micro PIXE analysis of arbuscular mycorrhizal fungal structures.•Chemical elements distribution maps.•Trace elements association with spores wall and polyphosphate granules.