Role of vitamin E on bovine skeletal-muscle-derived cells from Korean native cattle under heat treatment

• Published in: Journal of animal science 2024-01, Vol.102
• Main Authors: Kim, Bo-Mi, Jin, Xue-Cheng, Lee, Jun-Hee, Peng, Dong-Qiao, Kim, Won-Seob, Lee, Hong-Gu
• Format: Article
• Language: English
• Subjects: Animals; Antioxidants - metabolism; Antioxidants - pharmacology; Cattle; Cell Differentiation - drug effects; Cells, Cultured; Heat-Shock Response - drug effects; Hot Temperature - adverse effects; Muscle Development - drug effects; Muscle, Skeletal - drug effects; Republic of Korea; Vitamin E - pharmacology
• ISSN: 0021-8812; 1525-3163; 1525-3163
• DOI: 10.1093/jas/skae292

Our study aimed to evaluate the dualistic effect of heat stress on muscle differentiation at different temperatures, and whether vitamin E, a powerful antioxidant, could offset any negative effects, using bovine skeletal-muscle-derived cells (BSMCs) with myogenic properties. The BSMCs were extracted from the skeletal muscle of 30-mo-old Korean native cattle and subjected to myogenic differentiation under 3 heat exposure conditions: 37 °C (control; CON), 39 °C (mild heat stress; MHS), and 41 °C (severe heat stress; SHS) for 24 h with or without vitamin E treatment (NE or VE). After 24 h treatments, the cells were returned to 37 °C incubators and differentiated until day 6. On day 1, because of the heat exposure, the gene expression of MYOG was the highest in MHS (P = 0.047), suggesting a promotive effect of mild heat stress on myogenic differentiation, while on day 6, compared with CON and MHS, MYOD (P = 0.013) and MYOG (P = 0.029) were the lowest in SHS. Vitamin E treatment also lowered MYOG (P = 0.097), regardless of heat exposure. On day 1, HSPB1 (P = 0.001) and HSP70 (P