Microbiological studies on Escherichia coli and Klebsiella pneumoniae causing vaginal and urinary tract inflammation; prevalence, antibiotics-resistance and natural products susceptibility

• Published in: Microbial pathogenesis 2024-11, Vol.196, p.107000, Article 107000
• Main Authors: Soliman, Azza M., Shaaban, Mohamed T., Dawood, Aymen S., Shaheen, Mohamed N., Salama, Hussein S.
• Format: Article
• Language: English
• Subjects: Antimicrobial activity; Apple cider vinegar; Multidrug-resistant bacteria; Natural essential oil; Vaginal and urinary tract infection
• ISSN: 0882-4010; 1096-1208; 1096-1208
• DOI: 10.1016/j.micpath.2024.107000

Great efforts have been made for controlling multidrug-resistant bacteria (MDR). The antibacterial activity of natural products is an effective strategy due to its beneficial effects on human health. This study focused on the isolation, identification, studying the prevalence and the susceptibility of Escherichia coli and Klebsiella spp. to some natural products. After isolation, the isolates primarily identified using biochemical and API tests. Then, the antibiotic susceptibility test was done to reveal the most antibiotics resistant isolates before further identification of Escherichia coli and Klebsiella pneumoniae using phoA and gyrA genes, respectively. Furthermore, the sensitivity of the most antibiotics resistant isolates to apple cider vinegar (ACV), garlic oil (GO), coconut oil (CNO), tea tree oil (TTO), and lavender oil (LO) was estimated. All tested extracts especially ACV and TTO showed good antibacterial activity against MRD selected isolates. Statistical analysis indicated that there was significant difference in ACV and TTO antibacterial response between Escherichia coli and Klebsiella pneumoniae whereas there was no significant difference between natural products activity when tested against Escherichia coli and Klebsiella pneumoniae seperatly. GC-MS spectroscopy analysis revealed the most prominent active constituents present in tested ACV and TTO. Analyzing the 16S rRNA sequence confirmed the two most MRD pathogenic isolates that showed sensitivity to ACV and TTO were Escherichia coli MS1 and Klebsiella pneumoniae MS47, respectively. Essential oils tea tree and apple cider vinegar showed good antibacterial activities against antibiotics-resistant Escherichia coli and Klebsiella pneumoniae causing vaginal and urinary tract inflammation. •Prevalence of Escherichia coli and Klebsiella pneumoniae causing vaginal and urinary tract inflammation.•Increasing the presence of multidrug-resistant Escherichia coli and Klebsiella pneumoniae in Egypt.•Efficiency of natural products against MDR Escherichia coli and Klebsiella pneumoniae.•Excellent antibacterial activity of applecider vinegar and tea tree oil against isolated MDR bacteria.