Endemic maintenance of human-related hepatitis E virus strains in synurbic wild boars, Barcelona Metropolitan Area, Spain

• Published in: The Science of the total environment 2024-12, Vol.955, p.176871, Article 176871
• Main Authors: Ruiz-Ponsell, Laia, Monastiri, Abir, López-Roig, Marc, Sauleda, Sílvia, Bes, Marta, Mentaberre, Gregorio, Escobar-González, María, Costafreda, Maria I., López-Olvera, Jorge R., Serra-Cobo, Jordi
• Format: Article
• Language: English
• Subjects: Animals; Feces - virology; Genotype; Hepatitis E - epidemiology; Hepatitis E - veterinary; Hepatitis E - virology; Hepatitis E virus - genetics; Hepatitis E virus - isolation & purification; HEV; Humans; One health; Phylogeny; Public health; Spain; Sus scrofa; Swine; Swine Diseases - epidemiology; Swine Diseases - virology; Synurbization; Zoonoses - virology; Zoonosis
• ISSN: 0048-9697; 1879-1026; 1879-1026
• DOI: 10.1016/j.scitotenv.2024.176871

Hepatitis E virus (HEV), shared by humans, domestic animals, and wildlife, is an emerging global public health threat. Because wild boars are a major reservoir of HEV, the new zoonotic interfaces resulting from wild boar population increase and synurbization significantly contribute to increasing the risk of zoonotic transmission of HEV. This study characterizes HEV strains of synurbic wild boars and assesses their relationship with sympatric human and domestic swine HEV strains. We analyzed the faeces of 312 synurbic wild boars collected from 2016 to 2021 in the Barcelona Metropolitan Area (BMA), where there is a high density of wild boars, and found 7 HEV-positive samples among those collected between 2019 and 2020. The molecular analysis of these isolates, along with 6 additional wild boar HEV isolates from a previous study, allowed us to establish a close phylogenetic relationship between these HEV strains and human HEV isolates from sympatric blood donors and domestic pigs from Catalonia. HEV-positive wild boar samples belonged to piglet, juvenile and yearling individuals, but not adults, indicating the endemic maintenance of HEV in the wild boar population of the BMA by naïve young individuals. All wild boar HEV isolates in this study classified within HEV genotype 3. The results show, for the first time, a close molecular similarity between the HEV strains endemically maintained by the synurbic wild boars in the BMA and citizens from the same area and period. The data could also indicate that HEV infection presents a seasonal and interannual variability in wild boars of BMA. Further investigation is required to unveil the HEV transmission routes between synurbic wild boars and sympatric citizens. These findings can serve in other synurbic wildlife-human interfaces throughout the world. [Display omitted] •Hepatitis E virus (HEV) is an emerging global public health threat.•Synurbic wild boars can carry and release HEV into urban environments.•HEV was detected in piglets, juvenile and yearling wild boars, but not in adults.•HEV strains infecting wild boars from Barcelona in 2016–2021 belonged to genotype 3.•The wild boar HEV strains were closely related to those infecting BMA citizens.