Nisin-loaded chitosan/sodium alginate microspheres enhance the antimicrobial efficacy of nisin against Staphylococcus aureus

To develop and evaluate nisin-loaded chitosan/sodium alginate (CS/SA) microspheres as an improved antimicrobial delivery system targeting Staphylococcus aureus strains. The microspheres were prepared using a modified water-in-oil emulsion cross-linking method, resulting in spherical particles sized...

Full description

Saved in:
Bibliographic Details
Published in:Journal of applied microbiology 2024-11, Vol.135 (11)
Main Authors: Tang, Taya, Chen, Yinzhu, Zhao, Zhongling, Bai, Qianyu, Leisner, Jørgen J, Liu, Tianlong
Format: Article
Language:English
Subjects:
Alginates - chemistry
Anti-Bacterial Agents - pharmacology
Chitosan - chemistry
Chitosan - pharmacology
Glucuronic Acid - chemistry
Glucuronic Acid - pharmacology
Hexuronic Acids - chemistry
Hexuronic Acids - pharmacology
Microbial Sensitivity Tests
Microscopy, Electron, Scanning
Microspheres
Nisin - pharmacology
Particle Size
Staphylococcus aureus - drug effects
Staphylococcus aureus - growth & development
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites cdi_FETCH-LOGICAL-c180t-67ff7578eed0c95aae7e42b85ff81f433ac47b197c562943b9057282759937ee3
container_end_page
container_issue 11
container_start_page
container_title Journal of applied microbiology
container_volume 135
creator Tang, Taya
Chen, Yinzhu
Zhao, Zhongling
Bai, Qianyu
Leisner, Jørgen J
Liu, Tianlong
description To develop and evaluate nisin-loaded chitosan/sodium alginate (CS/SA) microspheres as an improved antimicrobial delivery system targeting Staphylococcus aureus strains. The microspheres were prepared using a modified water-in-oil emulsion cross-linking method, resulting in spherical particles sized 1-8 µm with a surface charge of -7.92 ± 5.09 mV, confirmed by scanning electron microscopy (SEM) and Zetasizer analysis. Encapsulation efficiency (EE) and loading capacity (LC) of nisin were 87.60% ± 0.43% and 1.99% ± 0.01%, respectively. In vitro release studies over 48 h indicated a controlled release pattern of nisin, described by the Korsmeyer-Peppas model, with higher release rates at 37°C and alkaline pH. Antimicrobial assays showed an enhanced efficacy of nisin-loaded CS/SA microspheres compared to free nisin, with minimum inhibitory concentration values reduced by 50%. Confocal laser scanning microscopy (CLSM), SEM, and transmission electron microscopy showed significant bacterial membrane damage and cellular disruption induced by the microspheres. This study highlights the potential of nisin-loaded CS/SA microspheres as an innovative antimicrobial delivery system with improved stability and antimicrobial efficacy against S. aureus, addressing limitations associated with nisin applied alone.
doi_str_mv 10.1093/jambio/lxae259
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_3115968810</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3115968810</sourcerecordid><originalsourceid>FETCH-LOGICAL-c180t-67ff7578eed0c95aae7e42b85ff81f433ac47b197c562943b9057282759937ee3</originalsourceid><addsrcrecordid>eNpNkE1LxDAQhoMofl89So5e6iZNkzRHEb9A9KCeyzSdbCNtsjYtuOCPd9ddxdMMzPO-MA8hZ5xdcmbE7B362sdZ9wmYS7NDDrlQMsuVznf_7QfkKKV3xrhgUu2TA2GEKZRSh-TryScfsi5Cgw21rR9jgjBLsfFTT6Gb-wAj0t7bIaZFiwMmiqGFYJGOLVIIo_851h46is55C3ZJo6NhXUxhDj6kkb6MsGiXXbTR2ilRmAac0gnZc9AlPN3OY_J2e_N6fZ89Pt89XF89ZpaXbMyUdk5LXSI2zBoJgBqLvC6lcyV3hRBgC11zo61UuSlEbZjUeZlraYzQiOKYXGx6F0P8mDCNVe-Txa6DgHFKleBcGlWWnK3Qyw26_jcN6KrF4HsYlhVn1dp4tTFebY2vAufb7qnusfnDfxWLb0oCgdo</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3115968810</pqid></control><display><type>article</type><title>Nisin-loaded chitosan/sodium alginate microspheres enhance the antimicrobial efficacy of nisin against Staphylococcus aureus</title><source>Oxford Journals Online</source><source>Alma/SFX Local Collection</source><creator>Tang, Taya ; Chen, Yinzhu ; Zhao, Zhongling ; Bai, Qianyu ; Leisner, Jørgen J ; Liu, Tianlong</creator><creatorcontrib>Tang, Taya ; Chen, Yinzhu ; Zhao, Zhongling ; Bai, Qianyu ; Leisner, Jørgen J ; Liu, Tianlong</creatorcontrib><description>To develop and evaluate nisin-loaded chitosan/sodium alginate (CS/SA) microspheres as an improved antimicrobial delivery system targeting Staphylococcus aureus strains. The microspheres were prepared using a modified water-in-oil emulsion cross-linking method, resulting in spherical particles sized 1-8 µm with a surface charge of -7.92 ± 5.09 mV, confirmed by scanning electron microscopy (SEM) and Zetasizer analysis. Encapsulation efficiency (EE) and loading capacity (LC) of nisin were 87.60% ± 0.43% and 1.99% ± 0.01%, respectively. In vitro release studies over 48 h indicated a controlled release pattern of nisin, described by the Korsmeyer-Peppas model, with higher release rates at 37°C and alkaline pH. Antimicrobial assays showed an enhanced efficacy of nisin-loaded CS/SA microspheres compared to free nisin, with minimum inhibitory concentration values reduced by 50%. Confocal laser scanning microscopy (CLSM), SEM, and transmission electron microscopy showed significant bacterial membrane damage and cellular disruption induced by the microspheres. This study highlights the potential of nisin-loaded CS/SA microspheres as an innovative antimicrobial delivery system with improved stability and antimicrobial efficacy against S. aureus, addressing limitations associated with nisin applied alone.</description><identifier>ISSN: 1365-2672</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1093/jambio/lxae259</identifier><identifier>PMID: 39394666</identifier><language>eng</language><publisher>England</publisher><subject>Alginates - chemistry ; Anti-Bacterial Agents - pharmacology ; Chitosan - chemistry ; Chitosan - pharmacology ; Glucuronic Acid - chemistry ; Glucuronic Acid - pharmacology ; Hexuronic Acids - chemistry ; Hexuronic Acids - pharmacology ; Microbial Sensitivity Tests ; Microscopy, Electron, Scanning ; Microspheres ; Nisin - pharmacology ; Particle Size ; Staphylococcus aureus - drug effects ; Staphylococcus aureus - growth &amp; development</subject><ispartof>Journal of applied microbiology, 2024-11, Vol.135 (11)</ispartof><rights>The Author(s) 2024. Published by Oxford University Press on behalf of Applied Microbiology International.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c180t-67ff7578eed0c95aae7e42b85ff81f433ac47b197c562943b9057282759937ee3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39394666$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tang, Taya</creatorcontrib><creatorcontrib>Chen, Yinzhu</creatorcontrib><creatorcontrib>Zhao, Zhongling</creatorcontrib><creatorcontrib>Bai, Qianyu</creatorcontrib><creatorcontrib>Leisner, Jørgen J</creatorcontrib><creatorcontrib>Liu, Tianlong</creatorcontrib><title>Nisin-loaded chitosan/sodium alginate microspheres enhance the antimicrobial efficacy of nisin against Staphylococcus aureus</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>To develop and evaluate nisin-loaded chitosan/sodium alginate (CS/SA) microspheres as an improved antimicrobial delivery system targeting Staphylococcus aureus strains. The microspheres were prepared using a modified water-in-oil emulsion cross-linking method, resulting in spherical particles sized 1-8 µm with a surface charge of -7.92 ± 5.09 mV, confirmed by scanning electron microscopy (SEM) and Zetasizer analysis. Encapsulation efficiency (EE) and loading capacity (LC) of nisin were 87.60% ± 0.43% and 1.99% ± 0.01%, respectively. In vitro release studies over 48 h indicated a controlled release pattern of nisin, described by the Korsmeyer-Peppas model, with higher release rates at 37°C and alkaline pH. Antimicrobial assays showed an enhanced efficacy of nisin-loaded CS/SA microspheres compared to free nisin, with minimum inhibitory concentration values reduced by 50%. Confocal laser scanning microscopy (CLSM), SEM, and transmission electron microscopy showed significant bacterial membrane damage and cellular disruption induced by the microspheres. This study highlights the potential of nisin-loaded CS/SA microspheres as an innovative antimicrobial delivery system with improved stability and antimicrobial efficacy against S. aureus, addressing limitations associated with nisin applied alone.</description><subject>Alginates - chemistry</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Chitosan - chemistry</subject><subject>Chitosan - pharmacology</subject><subject>Glucuronic Acid - chemistry</subject><subject>Glucuronic Acid - pharmacology</subject><subject>Hexuronic Acids - chemistry</subject><subject>Hexuronic Acids - pharmacology</subject><subject>Microbial Sensitivity Tests</subject><subject>Microscopy, Electron, Scanning</subject><subject>Microspheres</subject><subject>Nisin - pharmacology</subject><subject>Particle Size</subject><subject>Staphylococcus aureus - drug effects</subject><subject>Staphylococcus aureus - growth &amp; development</subject><issn>1365-2672</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNpNkE1LxDAQhoMofl89So5e6iZNkzRHEb9A9KCeyzSdbCNtsjYtuOCPd9ddxdMMzPO-MA8hZ5xdcmbE7B362sdZ9wmYS7NDDrlQMsuVznf_7QfkKKV3xrhgUu2TA2GEKZRSh-TryScfsi5Cgw21rR9jgjBLsfFTT6Gb-wAj0t7bIaZFiwMmiqGFYJGOLVIIo_851h46is55C3ZJo6NhXUxhDj6kkb6MsGiXXbTR2ilRmAac0gnZc9AlPN3OY_J2e_N6fZ89Pt89XF89ZpaXbMyUdk5LXSI2zBoJgBqLvC6lcyV3hRBgC11zo61UuSlEbZjUeZlraYzQiOKYXGx6F0P8mDCNVe-Txa6DgHFKleBcGlWWnK3Qyw26_jcN6KrF4HsYlhVn1dp4tTFebY2vAufb7qnusfnDfxWLb0oCgdo</recordid><startdate>20241104</startdate><enddate>20241104</enddate><creator>Tang, Taya</creator><creator>Chen, Yinzhu</creator><creator>Zhao, Zhongling</creator><creator>Bai, Qianyu</creator><creator>Leisner, Jørgen J</creator><creator>Liu, Tianlong</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20241104</creationdate><title>Nisin-loaded chitosan/sodium alginate microspheres enhance the antimicrobial efficacy of nisin against Staphylococcus aureus</title><author>Tang, Taya ; Chen, Yinzhu ; Zhao, Zhongling ; Bai, Qianyu ; Leisner, Jørgen J ; Liu, Tianlong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c180t-67ff7578eed0c95aae7e42b85ff81f433ac47b197c562943b9057282759937ee3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Alginates - chemistry</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Chitosan - chemistry</topic><topic>Chitosan - pharmacology</topic><topic>Glucuronic Acid - chemistry</topic><topic>Glucuronic Acid - pharmacology</topic><topic>Hexuronic Acids - chemistry</topic><topic>Hexuronic Acids - pharmacology</topic><topic>Microbial Sensitivity Tests</topic><topic>Microscopy, Electron, Scanning</topic><topic>Microspheres</topic><topic>Nisin - pharmacology</topic><topic>Particle Size</topic><topic>Staphylococcus aureus - drug effects</topic><topic>Staphylococcus aureus - growth &amp; development</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tang, Taya</creatorcontrib><creatorcontrib>Chen, Yinzhu</creatorcontrib><creatorcontrib>Zhao, Zhongling</creatorcontrib><creatorcontrib>Bai, Qianyu</creatorcontrib><creatorcontrib>Leisner, Jørgen J</creatorcontrib><creatorcontrib>Liu, Tianlong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tang, Taya</au><au>Chen, Yinzhu</au><au>Zhao, Zhongling</au><au>Bai, Qianyu</au><au>Leisner, Jørgen J</au><au>Liu, Tianlong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nisin-loaded chitosan/sodium alginate microspheres enhance the antimicrobial efficacy of nisin against Staphylococcus aureus</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2024-11-04</date><risdate>2024</risdate><volume>135</volume><issue>11</issue><issn>1365-2672</issn><eissn>1365-2672</eissn><abstract>To develop and evaluate nisin-loaded chitosan/sodium alginate (CS/SA) microspheres as an improved antimicrobial delivery system targeting Staphylococcus aureus strains. The microspheres were prepared using a modified water-in-oil emulsion cross-linking method, resulting in spherical particles sized 1-8 µm with a surface charge of -7.92 ± 5.09 mV, confirmed by scanning electron microscopy (SEM) and Zetasizer analysis. Encapsulation efficiency (EE) and loading capacity (LC) of nisin were 87.60% ± 0.43% and 1.99% ± 0.01%, respectively. In vitro release studies over 48 h indicated a controlled release pattern of nisin, described by the Korsmeyer-Peppas model, with higher release rates at 37°C and alkaline pH. Antimicrobial assays showed an enhanced efficacy of nisin-loaded CS/SA microspheres compared to free nisin, with minimum inhibitory concentration values reduced by 50%. Confocal laser scanning microscopy (CLSM), SEM, and transmission electron microscopy showed significant bacterial membrane damage and cellular disruption induced by the microspheres. This study highlights the potential of nisin-loaded CS/SA microspheres as an innovative antimicrobial delivery system with improved stability and antimicrobial efficacy against S. aureus, addressing limitations associated with nisin applied alone.</abstract><cop>England</cop><pmid>39394666</pmid><doi>10.1093/jambio/lxae259</doi></addata></record>
fulltext fulltext
identifier ISSN: 1365-2672
ispartof Journal of applied microbiology, 2024-11, Vol.135 (11)
issn 1365-2672
1365-2672
language eng
recordid cdi_proquest_miscellaneous_3115968810
source Oxford Journals Online; Alma/SFX Local Collection
subjects Alginates - chemistry
Anti-Bacterial Agents - pharmacology
Chitosan - chemistry
Chitosan - pharmacology
Glucuronic Acid - chemistry
Glucuronic Acid - pharmacology
Hexuronic Acids - chemistry
Hexuronic Acids - pharmacology
Microbial Sensitivity Tests
Microscopy, Electron, Scanning
Microspheres
Nisin - pharmacology
Particle Size
Staphylococcus aureus - drug effects
Staphylococcus aureus - growth & development
title Nisin-loaded chitosan/sodium alginate microspheres enhance the antimicrobial efficacy of nisin against Staphylococcus aureus
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-28T12%3A33%3A09IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Nisin-loaded%20chitosan/sodium%20alginate%20microspheres%20enhance%20the%20antimicrobial%20efficacy%20of%20nisin%20against%20Staphylococcus%20aureus&rft.jtitle=Journal%20of%20applied%20microbiology&rft.au=Tang,%20Taya&rft.date=2024-11-04&rft.volume=135&rft.issue=11&rft.issn=1365-2672&rft.eissn=1365-2672&rft_id=info:doi/10.1093/jambio/lxae259&rft_dat=%3Cproquest_cross%3E3115968810%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c180t-67ff7578eed0c95aae7e42b85ff81f433ac47b197c562943b9057282759937ee3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=3115968810&rft_id=info:pmid/39394666&rfr_iscdi=true