Apelin‐13's Actions in Controlling Hypertension‐Related Cardiac Hypertrophy and the Expressions of Inflammatory Cytokines

• Published in: Chemical biology & drug design 2024-10, Vol.104 (4), p.e14628-n/a
• Main Authors: Wei, Xiaoliang, Luo, Liyun, Lu, Huifang, Li, Songbiao, Deng, Xinlian, Li, Zhihui, Gong, Dong, Chen, Bairong
• Format: Article
• Language: English
• Subjects: Animals; Apelin‐13; Cardiomegaly - drug therapy; Cardiomegaly - metabolism; Cardiomegaly - pathology; Cell Line; Cytokines - metabolism; Disease Models, Animal; Hippo pathway; hypertension; Hypertension - drug therapy; Hypertension - metabolism; Inflammation - drug therapy; Inflammation - metabolism; Intercellular Signaling Peptides and Proteins - metabolism; Male; Mice; Mice, Inbred C57BL; myocardial inflammation; Myocytes, Cardiac - drug effects; Myocytes, Cardiac - metabolism; Myocytes, Cardiac - pathology; pyroptosis; Pyroptosis - drug effects; Rats; Signal Transduction - drug effects
• ISSN: 1747-0277; 1747-0285; 1747-0285
• DOI: 10.1111/cbdd.14628

ABSTRACT As a key molecule for improving cardiovascular diseases, Apelin‐13 was surveyed in this work to explain its actions in controlling inflammation, pyroptosis, and myocardial hypertrophy. First, mouse models with myocardial hypertrophy were established. Then, assessments were made on the pathological variation in the heart of mouse, on the cardiac functions, as well as on the expressions of cardiac hypertrophy markers (β‐MHC, ANP, and BNP), inflammatory factors (TNF‐α, COX2, IL‐6, ICAM‐1, and VCAM‐1), myocardial cell pyroptosis markers (NLRP3, ASC, c‐caspase‐1, and GSDMD‐N), and Hippo pathway proteins (p‐YAP, YAP, LATS1, and p‐LATS1) by HE staining, echocardiography scanning, and western blot tests separately. The expressions of such inflammatory factors as in myocardial tissue were acquired by ELISA. After inducing the phenotype of H9c2 cell hypertrophy by noradrenaline, we used CCK‐8 kits to know about the activity of H9c2 cells treated with Apelin‐13, and performed ɑ‐actinin staining to measure the changes in volumes of such cells. As unraveled through this work, Apelin‐13 refrained the activation of the Hippo pathway, which in turn attenuated the hypertrophy, inflammation, and pyroptosis of myocardial tissue and H9c2 cells. Hence, Apelin‐13 can be considered as a target for hypertension treatment. Apelin‐13 can inhibit the activation of the Hippo pathway and alleviate the inflammatory cascade in TAC mice myocardial tissue and NE‐induced H9c2 cells, improving cell pyroptosis, myocardial hypertrophy, and cardiac function.