One-pot synthesis of monodisperse silver-lignin particles: Enhanced antibacterial agents against antibiotic-resistant bacteria

• Published in: International journal of biological macromolecules 2024-11, Vol.281 (Pt 4), p.136552, Article 136552
• Main Authors: You, Sang-Mook, Kang, Dong-Gook, Choi, June-Ho, Kim, Younghoon, Jang, Hyeon Soo, Jung, Chan-Duck, Seong, Hyolin, Kim, Young-Rok, Cha, Hyun Gil, Kim, Hoyong
• Format: Article
• Language: English
• Subjects: Anti-Bacterial Agents - chemical synthesis; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Antibacterial; Antibiotic-resistant bacteria; Drug Resistance, Bacterial - drug effects; Green fabrication; Lignin; Lignin - chemistry; Lignin - pharmacology; Metal Nanoparticles - chemistry; Microbial Sensitivity Tests; One-pot strategy; Particle Size; Poly-l-lysine; Polylysine - chemistry; Silver - chemistry; Silver - pharmacology
• ISSN: 0141-8130; 1879-0003; 1879-0003
• DOI: 10.1016/j.ijbiomac.2024.136552

Lignin-based supports for metal nanoparticles (NPs) have attracted significant attention due to their abundant functional groups that facilitate NPs loading. However, many studies involve a two-step process: fabricating lignin particles and then reducing metal ions to NPs using physical energy consumption or chemical reduction. A one-step in-situ reduction method for NP synthesis on carrier surfaces, eliminating energy consumption, is needed for environmentally friendly and sustainable approach. Herein, we demonstrate that poly-l-lysine (PL) controls the self-assembly kinetics of kraft lignin (KL), and reduces silver ion (Ag+) to silver nanoparticles (AgNPs), forming highly monodisperse, co-self-assembled PL-KL particles (Ag@PL-KLPs) without chemical reducing agents or energy consumption. PL facilitated rapid KL desolvation, promoting intermolecular interactions and silver ion adsorption, followed by an efficient, separate nucleation and growth process yielded Ag@PL-KLPs approximately 270 nm in size with a narrow distribution. Notably, Ag@PL-KLPs exhibited enhanced bacteriostatic and bactericidal properties against antibiotic-resistant bacteria (ARB), including both Gram-negative and Gram-positive strains, at concentrations of 250 μg/mL. Leveraging biomass-derived lignin and this cost-effective, one-step green synthesis approach offers a sustainable method for avoiding antibiotic overuse and environmental contamination. [Display omitted] •Biomass-based one-step fabrication for antibacterial agent was established.•Poly-l-lysine modulate lignin self-assembly kinetics and enhance AgNPs reduction.•Monodisperse Ag@PL-KLPs exhibit enhanced antibacterial activity.•Breakthrough for reducing pollution from antibacterial agents' synthesis.