Single-molecule tracking technology based on symmetric power exponential phases

We present a design approach for a symmetric power exponential phase, resulting in a phase-modulated point-spread function with two symmetrical mainlobes that can be steered toward the optical axis opposite the x axis. This design offers nanoscale 3D localization capabilities suitable for 3D single-...

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Bibliographic Details
Published in:Optics letters 2024-10, Vol.49 (20), p.5870
Main Authors: Wang, Famin, Liu, Huijian, Zhang, Yunhai, Ge, Zhou, Li, Jiao, Wen, Gang, Xiao, Yun, Wang, Chi
Format: Article
Language:English
Subjects:
Algorithms
Localization
Numerical aperture
Optimization
Point spread functions
Sidelobes
Tracking
Transfer functions
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Summary:We present a design approach for a symmetric power exponential phase, resulting in a phase-modulated point-spread function with two symmetrical mainlobes that can be steered toward the optical axis opposite the x axis. This design offers nanoscale 3D localization capabilities suitable for 3D single-molecule tracking and localization imaging. The axial probing depth of this symmetric power exponential point-spread function can be adjusted as needed by manipulating specific parameters. Optimization of the symmetric power exponential phase involves truncation filtering to reduce sidelobes and the utilization of a phase inversion-based optimization algorithm to enhance transfer function efficiency and localization precision. A successful multi-molecule 3D tracking experiment was conducted at a 10 µm axial depth using a numerical aperture of 1.4 to validate the efficacy of the proposed design methodology.
ISSN:0146-9592
1539-4794
1539-4794
DOI:10.1364/OL.537258