The hippocampal CA2 region discriminates social threat from social safety

• Published in: Nature neuroscience 2024-11, Vol.27 (11), p.2193-2206
• Main Authors: Kassraian, Pegah, Bigler, Shivani K., Gilly Suarez, Diana M., Shrotri, Neilesh, Barnett, Anastasia, Lee, Heon-Jin, Young, W. Scott, Siegelbaum, Steven A.
• Format: Article
• Language: English
• Subjects: 14/19; 14/34; 631/378/2645; 631/378/2649; 631/378/87; 64/60; Ammon's horn; Animal Genetics and Genomics; Animals; Behavior; Behavioral Sciences; Biological Techniques; Biomedical and Life Sciences; Biomedicine; CA2 Region, Hippocampal - physiology; Calcium imaging; Conditioning, Classical - physiology; Conspecifics; Fear; Fear - physiology; Female; Females; Hippocampus; Male; Memory; Mice; Mice, Inbred C57BL; Neurobiology; Neurons; Neurosciences; Pyramidal cells; Pyramidal Cells - physiology; Recall; Representations; Safety; Social Behavior; Social interactions; Surgeons
• ISSN: 1097-6256; 1546-1726; 1546-1726
• DOI: 10.1038/s41593-024-01771-8

The dorsal cornu ammonis 2 (dCA2) region of the hippocampus enables the discrimination of novel from familiar conspecifics. However, the neural bases for more complex social–spatial episodic memories are unknown. Here we report that the spatial and social contents of an aversive social experience require distinct hippocampal regions. While dorsal CA1 (dCA1) pyramidal neurons mediate the memory of an aversive location, dCA2 pyramidal neurons enable the discrimination of threat-associated (CS + ) from safety-associated (CS − ) conspecifics in both female and male mice. Silencing dCA2 during encoding or recall trials disrupted social fear discrimination memory, resulting in fear responses toward both the CS + and CS − mice. Calcium imaging revealed that the aversive experience strengthened and stabilized dCA2 representations of both the CS + and CS − mice, with the incorporation of an abstract representation of social valence into representations of social identity. Thus, dCA2 contributes to both social novelty detection and the adaptive discrimination of threat-associated from safety-associated individuals during an aversive social episodic experience. The hippocampal dorsal CA2 enables the recognition of novel conspecifics. Kassraian et al. show that it is also required for discriminating safety- versus threat-associated conspecifics and that its disruption gives rise to generalized social avoidance.