SIRT2-mediated deacetylation of glutathione transferase alleviates oxidative damage and increases the heat tolerance of Pleurotus ostreatus

• Published in: Environmental research 2024-12, Vol.263 (Pt 2), p.120147, Article 120147
• Main Authors: Li, Huihui, Chai, Qianqian, zheng, Xiukun, Wen, Qing, Liu, Qing, Qi, Yuan Cheng, Wang, Fengqin, Shen, Jinwen, Hu, Yanru
• Format: Article
• Language: English
• Subjects: Acetylation; Glutathione Transferase - genetics; Glutathione Transferase - metabolism; High-temperature stress; Hot Temperature - adverse effects; Oxidative Stress; Pleurotus - enzymology; Pleurotus - genetics; Pleurotus ostreatus; SIRT2; Sirtuin 2 - genetics; Sirtuin 2 - metabolism; Thermotolerance
• ISSN: 0013-9351; 1096-0953; 1096-0953
• DOI: 10.1016/j.envres.2024.120147

High-temperature stress (HS) severely threatens agricultural production. Pleurotus ostreatus is cultivated in many parts of the world, and its growth is strongly affected by HS. We previously reported that metabolic rearrangement occurred in HS, but the gene expression levels of several key enzymes remained unchanged. Therefore, in this study, we investigated the contribution of posttranslational modifications of proteins to HS resistance in P. ostreatus. We found that the level of acetylation of P. ostreatus decreased under short-term HS treatment and increased as the duration of HS treatment increased. Acetylation omics revealed that almost all metabolic enzymes were acetylated. We found that deacetylation under HS can improve the growth recovery ability of mycelia, the activity of matrix-degrading enzyme, and the contents of antioxidants, such as nicotinamide adenine dinucleotide phosphate (NADPH) and glutathione (GSH), but can decreased H2O2 levels. In vitro acetylation experiments and point mutations revealed that the deacetylase SIRT2 increased the activity of glutathione transferases (GSTs) by deacetylating GST1 66K, GST2 206K, and GST2 233K. Together, SIRT2 is activated by short-term HS and improves its antioxidant activity by deacetylating GSTs, thereby improving the resistance of P. ostreatus to HS. In this study, we identified new non-histone substrate proteins and new lysine acetylation sites of SIRT2 under HS. We also discovered the role of non-histone acetylation in the adaptation of organisms to HS. •The lysine acetylation under heat stress in macrofungi was reported firstly.•New non-histone substrate proteins of SIRT2 and the new lysine acetylation sites under heat stress were identified.•SIRT2 is activated by short-term heat stress and improves its antioxidant activity by deacetylating GSTs.