Identification of FGG as a Biomarker in Early Gastric Cancer via Tissue Proteomics and Clinical Verification

• Published in: Journal of proteome research 2024-11, Vol.23 (11), p.5122-5130
• Main Authors: Chen, Wujie, Ye, Qihua, Zhang, Biying, Ma, Zhenhua, Tu, Hanxiao
• Format: Article
• Language: English
• Subjects: Biomarkers, Tumor - analysis; Biomarkers, Tumor - metabolism; Early Detection of Cancer - methods; Female; Fibrinogen - analysis; Fibrinogen - metabolism; Gastric Mucosa - metabolism; Gastric Mucosa - pathology; Humans; Immunohistochemistry; Male; Middle Aged; Proteomics - methods; Stomach Neoplasms - diagnosis; Stomach Neoplasms - metabolism; Stomach Neoplasms - pathology; Up-Regulation
• ISSN: 1535-3893; 1535-3907; 1535-3907
• DOI: 10.1021/acs.jproteome.4c00624

Early and accurate diagnosis of gastric cancer (GC) is essential for reducing mortality and improving patient well-being. However, methods for the early diagnosis of GC are still lacking. In this study, by isobaric tagging for relative and absolute quantitation (iTRAQ), we identified 336 proteins that overlapped among the upregulated differentially expressed proteins (DEPs) in early gastric cancer (EGC) versus progressive gastric cancer (PGC), upregulated DEPs in EGC versus nongastric cancer (NGC), and nonsignificant proteins in EGC versus NGC. These DEPs were involved primarily in the neutrophil-related immune response. Network analysis of proteins and pathways revealed that fibrinogen α (FGA), β (FGB), and γ (FGG) are candidates for distinguishing EGC. Furthermore, parallel reaction monitoring (PRM), immunohistochemistry (IHC), and Western blot (WB) assays of clinical samples confirmed that, compared with that in PGC and NGC, only FGG was uniquely and significantly upregulated in the gastric mucosa of EGC. Our results demonstrated that FGG in the gastric mucosa could be a novel biomarker to diagnose EGC patients via endoscopy.