A Critical View on the Use of DNA Hydrogels in Cell-Free Protein Synthesis

Numerous studies have reported in the past that the use of protein-encoding DNA hydrogels as templates for cell-free protein synthesis (CFPS) leads to better yields than the use of conventional templates such as plasmids or PCR fragments. Systematic investigation of different types of bulk materials...

Full description

Saved in:
Bibliographic Details
Published in:Angewandte Chemie International Edition 2024-11, p.e202414480
Main Authors: Moench, Svenja, Lemke, Phillip, Hansen, Abbey, Bickmann, Christoph, Peng, Martin, Rabe, Kersten S, Domínguez, Carmen M, Niemeyer, Christof M
Format: Article
Language:English
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Numerous studies have reported in the past that the use of protein-encoding DNA hydrogels as templates for cell-free protein synthesis (CFPS) leads to better yields than the use of conventional templates such as plasmids or PCR fragments. Systematic investigation of different types of bulk materials from pure DNA hydrogels and DNA hydrogel composites using a commercially available CFPS kit showed no evidence of improved expression efficiency. However, protein-coding DNA hydrogels were advantageously used in microfluidic reactors as immobilized templates for repetitive protein production, suggesting that DNA-based materials offer potential for future developments in high-throughput profiling or rapid in situ characterization of proteins.
ISSN:1433-7851
1521-3773
1521-3773
DOI:10.1002/anie.202414480